LSBio
Human SULF2 / Sulfatase 2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human SULF2 / Sulfatase 2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
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$220.00each
Catalog Number: LS-G86997-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Sulfatase 2(SULF2) (NM_001161841) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Extracellular sulfatase Sulf-2 | KIAA1247 | Sulfatase 2 | HSULF-2 | SULF-2 | SULF2
Origin Species
Human
NCBI GenBank
AB033073
SwissProt
Q8IWU5

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
100.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGPPSLVLCL LSATVFSLLG GSSAFLSHHR LKGRFQRDRR NIRPNIILVL TDDQDVELGS
61MQVMNKTRRI MEQGGAHFIN AFVTTPMCCP SRSSILTGKY VHNHNTYTNN ENCSSPSWQA
121QHESRTFAVY LNSTGYRTAF FGKYLNEYNG SYVPPGWKEW VGLLKNSRFY NYTLCRNGVK
181EKHGSDYSKD YLTDLITNDS VSFFRTSKKM YPHRPVLMVI SHAAPHGPED SAPQYSRLFP
241NASQHITPSY NYAPNPDKHW IMRYTGPMKP IHMEFTNMLQ RKRLQTLMSV DDSMETIYNM
301LVETGELDNT YIVYTADHGY HIGQFGLVKG KSMPYEFDIR VPFYVRGPNV EAGCLNPHIV
361LNIDLAPTIL DIAGLDIPAD MDGKSILKLL DTERPVNRFH LKKKMRVWRD SFLVERGKLL
421HKRDNDKVDA QEENFLPKYQ RVKDLCQRAE YQTACEQLGQ KWQCVEDATG KLKLHKCKGP
481MRLGGSRALS NLVPKYYGQG SEACTCDSGD YKLSLAGRRK KLFKKKYKAS YVRSRSIRSV
541AIEVDGRVYH VGLGDAAQPR NLTKRHWPGA PEDQDDKDGG DFSGTGGLPD YSAANPIKVT
601HRCYILENDT VQCDLDLYKS LQAWKDHKLH IDHEIETLQN KIKNLREVRG HLKKKRPEEC
661DCHKISYHTQ HKGRLKHRGS SLHPFRKGLQ EKDKVWLLRE QKRKKKLRKL LKRLQNNDTC
721SMPGLTCFTH DNQHWQTAPF WTLGPFCACT SANNNTYWCM RTINETHNFL FCEFATGFLE
781YFDLNTDPYQ LMNAVNTLDR DVLNQLHVQL MELRSCKGYK QCNPRTRNMD LGLKDGGSYE
841QYRQFQRRKW PEMKRPSSKS LGQLWEGWEG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

870 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.