Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SULF2 / Sulfatase 2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G128801-100
Catalog Number: LS-G128801
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Sulfatase 2(SULF2) (NM_001161841), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Extracellular sulfatase Sulf-2 | KIAA1247 | Sulfatase 2 | HSULF-2 | SULF-2 | SULF2
- Origin Species
- Human
- NCBI GenBank
- AB033073
- SwissProt
- Q8IWU5
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 100.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGPPSLVLCL LSATVFSLLG GSSAFLSHHR LKGRFQRDRR NIRPNIILVL TDDQDVELGS61MQVMNKTRRI MEQGGAHFIN AFVTTPMCCP SRSSILTGKY VHNHNTYTNN ENCSSPSWQA121QHESRTFAVY LNSTGYRTAF FGKYLNEYNG SYVPPGWKEW VGLLKNSRFY NYTLCRNGVK181EKHGSDYSKD YLTDLITNDS VSFFRTSKKM YPHRPVLMVI SHAAPHGPED SAPQYSRLFP241NASQHITPSY NYAPNPDKHW IMRYTGPMKP IHMEFTNMLQ RKRLQTLMSV DDSMETIYNM301LVETGELDNT YIVYTADHGY HIGQFGLVKG KSMPYEFDIR VPFYVRGPNV EAGCLNPHIV361LNIDLAPTIL DIAGLDIPAD MDGKSILKLL DTERPVNRFH LKKKMRVWRD SFLVERGKLL421HKRDNDKVDA QEENFLPKYQ RVKDLCQRAE YQTACEQLGQ KWQCVEDATG KLKLHKCKGP481MRLGGSRALS NLVPKYYGQG SEACTCDSGD YKLSLAGRRK KLFKKKYKAS YVRSRSIRSV541AIEVDGRVYH VGLGDAAQPR NLTKRHWPGA PEDQDDKDGG DFSGTGGLPD YSAANPIKVT601HRCYILENDT VQCDLDLYKS LQAWKDHKLH IDHEIETLQN KIKNLREVRG HLKKKRPEEC661DCHKISYHTQ HKGRLKHRGS SLHPFRKGLQ EKDKVWLLRE QKRKKKLRKL LKRLQNNDTC721SMPGLTCFTH DNQHWQTAPF WTLGPFCACT SANNNTYWCM RTINETHNFL FCEFATGFLE781YFDLNTDPYQ LMNAVNTLDR DVLNQLHVQL MELRSCKGYK QCNPRTRNMD LGLKDGGSYE841QYRQFQRRKW PEMKRPSSKS LGQLWEGWEGTRTRPLEQKLISEEDLAANDILDYKDDDDKV
870 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.