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Human STKLD1 / SgK071 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human STKLD1 / SgK071 Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all STKLD1 antibodies →
$153.00each
Catalog Number: LS-G83116-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human C9orf96(STKLD1) (NM_153710) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C9orf96 | Gm711 | Sugen kinase 071 | Predicted gene 711 | SgK071 | Sk521 | RP11-244N20.8 | RP23-414L19.8
Origin Species
Human
NCBI GenBank
NM_153710
SwissProt
Q8NE28

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
75.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLGPGSNRRR PTQGERGPGS PGEPMEKYQV LYQLNPGALG VNLVVEEMET KVKHVIKQVE
61CMDDHYASQA LEELMPLLKL RHAHISVYQE LFITWNGEIS SLYLCLVMEF NELSFQEVIE
121DKRKAKKIID SEWMQNVLGQ VLDALEYLHH LDIIHRNLKP SNIILISSDH CKLQDLSSNV
181LMTDKAKWNI RAEEDPFRKS WMAPEALNFS FSQKSDIWSL GCIILDMTSC SFMDGTEAMH
241LRKSLRQSPG SLKAVLKTME EKQIPDVETF RNLLPLMLQI DPSDRITIKD VVHITFLRGS
301FKSSCVSLTL HRQMVPASIT DMLLEGNVAS ILEVMQKFSG WPEVQLRAMK RLLKMPADQL
361GLPWPPELVE VVVTTMELHD RVLDVQLCAC SLLLHLLGQA LVHHPEAKAP CNQAITSTLL
421SALQSHPEEE PLLVMVYSLL AITTTQESES LSEELQNAGL LEHILEHLNS SLESRDVCAS
481GLGLLWALLL DGIIVNKAPL EKVPDLISQV LATYPADGEM AEASCGVFWL LSLLGCIKEQ
541QFEQVVALLL QSIRLCQDRA LLVNNAYRGL ASLVKVSELA AFKVVVQEEG GSGLSLIKET
601YQLHRDDPEV VENVGMLLVH LASYEEILPE LVSSSMKALL QEIKERFTSS LVSDSSAFSK
661PGLPPGGSPQ LGCTTSGGLE
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

680 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.