Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
STKLD1 / SgK071 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G105706-100
Catalog Number: LS-G105706
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C9orf96(STKLD1) (NM_153710), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C9orf96 | Gm711 | Sugen kinase 071 | Predicted gene 711 | SgK071 | Sk521 | RP11-244N20.8 | RP23-414L19.8
- Origin Species
- Human
- NCBI GenBank
- NM_153710
- SwissProt
- Q8NE28
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 75.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MLGPGSNRRR PTQGERGPGS PGEPMEKYQV LYQLNPGALG VNLVVEEMET KVKHVIKQVE61CMDDHYASQA LEELMPLLKL RHAHISVYQE LFITWNGEIS SLYLCLVMEF NELSFQEVIE121DKRKAKKIID SEWMQNVLGQ VLDALEYLHH LDIIHRNLKP SNIILISSDH CKLQDLSSNV181LMTDKAKWNI RAEEDPFRKS WMAPEALNFS FSQKSDIWSL GCIILDMTSC SFMDGTEAMH241LRKSLRQSPG SLKAVLKTME EKQIPDVETF RNLLPLMLQI DPSDRITIKD VVHITFLRGS301FKSSCVSLTL HRQMVPASIT DMLLEGNVAS ILEVMQKFSG WPEVQLRAMK RLLKMPADQL361GLPWPPELVE VVVTTMELHD RVLDVQLCAC SLLLHLLGQA LVHHPEAKAP CNQAITSTLL421SALQSHPEEE PLLVMVYSLL AITTTQESES LSEELQNAGL LEHILEHLNS SLESRDVCAS481GLGLLWALLL DGIIVNKAPL EKVPDLISQV LATYPADGEM AEASCGVFWL LSLLGCIKEQ541QFEQVVALLL QSIRLCQDRA LLVNNAYRGL ASLVKVSELA AFKVVVQEEG GSGLSLIKET601YQLHRDDPEV VENVGMLLVH LASYEEILPE LVSSSMKALL QEIKERFTSS LVSDSSAFSK661PGLPPGGSPQ LGCTTSGGLETRTRPLEQKLISEEDLAANDILDYKDDDDKV
680 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.