LSBio
Human SRSF11 / SFRS11 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human SRSF11 / SFRS11 Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all SRSF11 antibodies →
$153.00each
Catalog Number: LS-G74577-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human SFRS11(SRSF11) (NM_004768) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DJ677H15.2 | NET2 | p54 | SFRS11 | Splicing factor p54 | SR splicing factor 11 | SRSF11
Origin Species
Human
NCBI GenBank
NM_004768
SwissProt
Q05519

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
53.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSNTTVVPST AGPGPSGGPG GGGGGGGGGG GTEVIQVTNV SPSASSEQMR TLFGFLGKID
61ELRLFPPDDS PLPVSSRVCF VKFHDPDSAV VAQHLTNTVF VDRALIVVPY AEGVIPDEAK
121ALSLLAPANA VAGLLPGGGL LPTPNPLTQI GAVPLAALGA PTLDPALAAL GLPGANLNSQ
181SLAADQLLKL MSTVDPKLNH VAAGLVSPSL KSDTSSKEIE EAMKRVREAQ SLISAAIEPD
241KKEEKRRHSR SRSRSRRRRT PSSSRHRRSR SRSRRRSHSK SRSRRRSKSP RRRRSHSRER
301GRRSRSTSKT RDKKKEDKEK KRSKTPPKSY STARRSRSAS RERRRRRSRS GTRSPKKPRS
361PKRKLSRSPS PRRHKKEKKK DKDKERSRDE RERSTSKKKK SKDKEKDRER KSESDKDVKV
421TRDYDEEEQG YDSEKEKKEE KKPIETGSPK TKECSVEKGT GDSLRESKVN GDDHHEEDMD
481MSD
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

483 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.