Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SRSF11 / SFRS11 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G116426-100
Catalog Number: LS-G116426
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SFRS11(SRSF11) (NM_004768), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DJ677H15.2 | NET2 | p54 | SFRS11 | Splicing factor p54 | SR splicing factor 11 | SRSF11
- Origin Species
- Human
- NCBI GenBank
- NM_004768
- SwissProt
- Q05519
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 53.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSNTTVVPST AGPGPSGGPG GGGGGGGGGG GTEVIQVTNV SPSASSEQMR TLFGFLGKID61ELRLFPPDDS PLPVSSRVCF VKFHDPDSAV VAQHLTNTVF VDRALIVVPY AEGVIPDEAK121ALSLLAPANA VAGLLPGGGL LPTPNPLTQI GAVPLAALGA PTLDPALAAL GLPGANLNSQ181SLAADQLLKL MSTVDPKLNH VAAGLVSPSL KSDTSSKEIE EAMKRVREAQ SLISAAIEPD241KKEEKRRHSR SRSRSRRRRT PSSSRHRRSR SRSRRRSHSK SRSRRRSKSP RRRRSHSRER301GRRSRSTSKT RDKKKEDKEK KRSKTPPKSY STARRSRSAS RERRRRRSRS GTRSPKKPRS361PKRKLSRSPS PRRHKKEKKK DKDKERSRDE RERSTSKKKK SKDKEKDRER KSESDKDVKV421TRDYDEEEQG YDSEKEKKEE KKPIETGSPK TKECSVEKGT GDSLRESKVN GDDHHEEDMD481MSDTRTRPLEQKLISEEDLAANDILDYKDDDDKV
483 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.