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Human SH3PXD2A / TKS5 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human SH3PXD2A / TKS5 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all SH3PXD2A antibodies →
$220.00each
Catalog Number: LS-G79866-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human FISH(SH3PXD2A) (NM_014631) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Adapter protein TKS5 | Adaptor protein TKS5 | FISH | KIAA0418 | SH3 and PX domains 2A | SH3 multiple domains protein 1 | SH3PXD2A | TSK5 | SH3 multiple domains 1 | SH3MD1 | TKS5
Origin Species
Human
NCBI GenBank
AX695698
SwissProt
Q5TCZ1

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
121.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLAYCVQDAT VVDVEKRRNP SKHYVYIINV TWSDSTSQTI YRRYSKFFDL QMQLLDKFPI
61EGGQKDPKQR IIPFLPGKIL FRRSHIRDVA VKRLKPIDEY CRALVRLPPH ISQCDEVFRF
121FEARPEDVNP PKEDYGSSKR KSVWLSSWAE SPKKDVTGAD ATAEPMILEQ YVVVSNYKKQ
181ENSELSLQAG EVVDVIEKNE SGWWFVSTSE EQGWVPATYL EAQNGTRDDS DINTSKTGEE
241EKYVTVQPYT SQSKDEIGFE KGVTVEVIRK NLEGWWYIRY LGKEGWAPAS YLKKAKDDLP
301TRKKNLAGPV EIIGNIMEIS NLLNKKASGD KETPPAEGEG HEAPIAKKEI SLPILCNASN
361GSAVGVPDRT VSRLAQGSPA VARIAPQRAQ ISSPNLRTRP PPRRESSLGF QLPKPPEPPS
421VEVEYYTIAE FQSCISDGIS FRGGQKAEVI DKNSGGWWYV QIGEKEGWAP ASYIDKRKKP
481NLSRRTSTLT RPKVPPPAPP SKPKEAEEGP TGASESQDSP RKLKYEEPEY DIPAFGFDSE
541PELSEEPVED RASGERRPAQ PHRPSPASSL QRARFKVGES SEDVALEEET IYENEGFRPY
601AEDTLSARGS SGDSDSPGSS SLSLTRKNSP KSGSPKSSSL LKLKAEKNAQ AEMGKNHSSA
661SFSSSITINT TCCSSSSSSS SSLSKTSGDL KPRSASDAGI RGTPKVRAKK DADANAGLTS
721CPRAKPSVRP KPFLNRAESQ SQEKMDISTL RRQLRPTGQL RGGLKGSKSE DSELPPQTAS
781EAPSEGSRRS SSDLITLPAT TPPCPTKKEW EGPATSYMTC SAYQKVQDSE ISFPAGVEVQ
841VLEKQESGWW YVRFGELEGW APSHYLVLDE NEQPDPSGKE LDTVPAKGRQ NEGKSDSLEK
901IERRVQALNT VNQSKKATPP IPSKPPGGFG KTSGTPAVKM RNGVRQVAVR PQSVFVSPPP
961KDNNLSCALR RNESLTATDG LRGVRRNSSF STARSAAAEA KGRLAERAAS QGSDSPLLPA
1021QRNSIPVSPV RPKPIEKSQF IHNNLKDVYV SIADYEGDEE TAGFQEGVSM EVLERNPNGW
1081WYCQILDGVK PFKGWVPSNY LEKKN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1105 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.