Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SH3PXD2A / TKS5 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G113818-100
Catalog Number: LS-G113818
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FISH(SH3PXD2A) (NM_014631), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Adapter protein TKS5 | Adaptor protein TKS5 | FISH | KIAA0418 | SH3 and PX domains 2A | SH3 multiple domains protein 1 | SH3PXD2A | TSK5 | SH3 multiple domains 1 | SH3MD1 | TKS5
- Origin Species
- Human
- NCBI GenBank
- AX695698
- SwissProt
- Q5TCZ1
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 121.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MLAYCVQDAT VVDVEKRRNP SKHYVYIINV TWSDSTSQTI YRRYSKFFDL QMQLLDKFPI61EGGQKDPKQR IIPFLPGKIL FRRSHIRDVA VKRLKPIDEY CRALVRLPPH ISQCDEVFRF121FEARPEDVNP PKEDYGSSKR KSVWLSSWAE SPKKDVTGAD ATAEPMILEQ YVVVSNYKKQ181ENSELSLQAG EVVDVIEKNE SGWWFVSTSE EQGWVPATYL EAQNGTRDDS DINTSKTGEE241EKYVTVQPYT SQSKDEIGFE KGVTVEVIRK NLEGWWYIRY LGKEGWAPAS YLKKAKDDLP301TRKKNLAGPV EIIGNIMEIS NLLNKKASGD KETPPAEGEG HEAPIAKKEI SLPILCNASN361GSAVGVPDRT VSRLAQGSPA VARIAPQRAQ ISSPNLRTRP PPRRESSLGF QLPKPPEPPS421VEVEYYTIAE FQSCISDGIS FRGGQKAEVI DKNSGGWWYV QIGEKEGWAP ASYIDKRKKP481NLSRRTSTLT RPKVPPPAPP SKPKEAEEGP TGASESQDSP RKLKYEEPEY DIPAFGFDSE541PELSEEPVED RASGERRPAQ PHRPSPASSL QRARFKVGES SEDVALEEET IYENEGFRPY601AEDTLSARGS SGDSDSPGSS SLSLTRKNSP KSGSPKSSSL LKLKAEKNAQ AEMGKNHSSA661SFSSSITINT TCCSSSSSSS SSLSKTSGDL KPRSASDAGI RGTPKVRAKK DADANAGLTS721CPRAKPSVRP KPFLNRAESQ SQEKMDISTL RRQLRPTGQL RGGLKGSKSE DSELPPQTAS781EAPSEGSRRS SSDLITLPAT TPPCPTKKEW EGPATSYMTC SAYQKVQDSE ISFPAGVEVQ841VLEKQESGWW YVRFGELEGW APSHYLVLDE NEQPDPSGKE LDTVPAKGRQ NEGKSDSLEK901IERRVQALNT VNQSKKATPP IPSKPPGGFG KTSGTPAVKM RNGVRQVAVR PQSVFVSPPP961KDNNLSCALR RNESLTATDG LRGVRRNSSF STARSAAAEA KGRLAERAAS QGSDSPLLPA1021QRNSIPVSPV RPKPIEKSQF IHNNLKDVYV SIADYEGDEE TAGFQEGVSM EVLERNPNGW1081WYCQILDGVK PFKGWVPSNY LEKKNTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1105 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.