RPS6KB1 / P70S6K / S6K Antibody
Catalog Number: LS-C484702-100
100 µL / $309.00
Antibody: Rabbit Polyclonal
Applications: Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human RPS6KB1 / P70S6K / S6K Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G78667-20
Catalog Number: LS-G78667
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human S6K1(RPS6KB1) (NM_003161) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- 70 kDa S6 kinase | p70 S6KA | p70-alpha | p70-S6K | p70 S6 kinase alpha | p70s6 kinase | S6K-beta-1 | STK14A | p70 ribosomal S6 kinase | p70 S6 kinase | alpha 1 | alpha 2 | p70 S6K-alpha | p70-S6K 1 | p70S6K | p70S6K1 | PS6K | Ribosomal protein S6 kinase I | S6K | S6K1 | p70 ribosomal S6 kinase alpha | p70(S6K)-alpha | RPS6KB1
- Origin Species
- Human
- NCBI GenBank
- NM_003161
- SwissProt
- P23443
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 59 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MRRRRRRDGF YPAPDFRDRE AEDMAGVFDI DLDQPEDAGS EDELEEGGQL NESMDHGGVG61PYELGMEHCE KFEISETSVN RGPEKIRPEC FELLRVLGKG GYGKVFQVRK VTGANTGKIF121AMKVLKKAMI VRNAKDTAHT KAERNILEEV KHPFIVDLIY AFQTGGKLYL ILEYLSGGEL181FMQLEREGIF MEDTACFYLA EISMALGHLH QKGIIYRDLK PENIMLNHQG HVKLTDFGLC241KESIHDGTVT HTFCGTIEYM APEILMRSGH NRAVDWWSLG ALMYDMLTGA PPFTGENRKK301TIDKILKCKL NLPPYLTQEA RDLLKKLLKR NAASRLGAGP GDAGEVQAHP FFRHINWEEL361LARKVEPPFK PLLQSEEDVS QFDSKFTRQT PVDSPDDSTL SESANQVFLG FTYVAPSVLE421SVKEKFSFEP KIRSPRRFIG SPRTPVSPVK FSPGDFWGRG ASASTANPQT PVEYPMETSG481IEQMDVTMSG EASAPLPIRQ PNSGPYKKQA FPMISKRPEH LRMNLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
525 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
