Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
RPS6KB1 / P70S6K / S6K Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G99980-100
Catalog Number: LS-G99980
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human S6K1(RPS6KB1) (NM_003161), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- 70 kDa S6 kinase | p70 S6KA | p70-alpha | p70-S6K | p70 S6 kinase alpha | p70s6 kinase | S6K-beta-1 | STK14A | p70 ribosomal S6 kinase | p70 S6 kinase | alpha 1 | alpha 2 | p70 S6K-alpha | p70-S6K 1 | p70S6K | p70S6K1 | PS6K | Ribosomal protein S6 kinase I | S6K | S6K1 | p70 ribosomal S6 kinase alpha | p70(S6K)-alpha | RPS6KB1
- NCBI GenBank
- NM_003161
- SwissProt
- P23443
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 59 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MRRRRRRDGF YPAPDFRDRE AEDMAGVFDI DLDQPEDAGS EDELEEGGQL NESMDHGGVG61PYELGMEHCE KFEISETSVN RGPEKIRPEC FELLRVLGKG GYGKVFQVRK VTGANTGKIF121AMKVLKKAMI VRNAKDTAHT KAERNILEEV KHPFIVDLIY AFQTGGKLYL ILEYLSGGEL181FMQLEREGIF MEDTACFYLA EISMALGHLH QKGIIYRDLK PENIMLNHQG HVKLTDFGLC241KESIHDGTVT HTFCGTIEYM APEILMRSGH NRAVDWWSLG ALMYDMLTGA PPFTGENRKK301TIDKILKCKL NLPPYLTQEA RDLLKKLLKR NAASRLGAGP GDAGEVQAHP FFRHINWEEL361LARKVEPPFK PLLQSEEDVS QFDSKFTRQT PVDSPDDSTL SESANQVFLG FTYVAPSVLE421SVKEKFSFEP KIRSPRRFIG SPRTPVSPVK FSPGDFWGRG ASASTANPQT PVEYPMETSG481IEQMDVTMSG EASAPLPIRQ PNSGPYKKQA FPMISKRPEH LRMNLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
525 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.