PRLHR / GPR10 Antibody
Catalog Number: LS-C497189
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Immunohistochemistry (general), Western Blot
Reactivity: Human, Mouse
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human PRLHR / GPR10 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G78186-20
Catalog Number: LS-G78186
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human GPR10(PRLHR) (NM_004248) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- G protein-coupled receptor 10 | G-protein coupled receptor 10 | GPR10 | GR3 | HGR3 | PrRP receptor | PrRPR | Uhr-1 | PRLHR
- Origin Species
- Human
- NCBI GenBank
- NM_004248
- SwissProt
- P49683
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 40.9 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MASSTTRGPR VSDLFSGLPP AVTTPANQSA EASAGNGSVA GADAPAVTPF QSLQLVHQLK61GLIVLLYSVV VVVGLVGNCL LVLVIARVRR LHNVTNFLIG NLALSDVLMC TACVPLTLAY121AFEPRGWVFG GGLCHLVFFL QPVTVYVSVF TLTTIAVDRY VVLVHPLRRR ISLRLSAYAV181LAIWALSAVL ALPAAVHTYH VELKPHDVRL CEEFWGSQER QRQLYAWGLL LVTYLLPLLV241ILLSYVRVSV KLRNRVVPGC VTQSQADWDR ARRRRTFCLL VVVVVVFAVC WLPLHVFNLL301RDLDPHAIDP YAFGLVQLLC HWLAMSSACY NPFIYAWLHD SFREELRKLL VAWPRKIAPH361GQNMTVSVVITRTRPLEQKLISEEDLAANDILDYKDDDDKV
370 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
