PPP2R2B Antibody
Catalog Number: LS-C376380
2 sizes / from $300.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human PPP2R2B Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G78367-20
Catalog Number: LS-G78367
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human PPP2R2B (NM_181678) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- B55BETA | PP2ABBETA | PP2APR55BETA | PP2A subunit B isoform beta | PR2AB55BETA | PR55-BETA | SCA12 | Spinocerebellar ataxia 12 | PP2A subunit B isoform R2-beta | PP2AB55BETA | PP2APR55B | PR55BETA | PPP2R2B | PR2ABBETA | PR2APR55BETA | PR52B
- Origin Species
- Human
- NCBI GenBank
- NM_004576
- SwissProt
- Q00005
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 50.2 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MNYPDENTYG NKADIISTVE FNHTGELLAT GDKGGRVVIF QREQESKNQV HRRGEYNVYS61TFQSHEPEFD YLKSLEIEEK INKIRWLPQQ NAAYFLLSTN DKTVKLWKVS ERDKRPEGYN121LKDEEGRLRD PATITTLRVP VLRPMDLMVE ATPRRVFANA HTYHINSISV NSDYETYMSA181DDLRINLWNF EITNQSFNIV DIKPANMEEL TEVITAAEFH PHHCNTFVYS SSKGTIRLCD241MRASALCDRH TKFFEEPEDP SNRSFFSEII SSISDVKFSH SGRYIMTRDY LTVKVWDLNM301ENRPIETYQV HDYLRSKLCS LYENDCIFDK FECVWNGSDS VIMTGSYNNF FRMFDRNTKR361DVTLEASREN SKPRAILKPR KVCVGGKRRK DEISVDSLDF SKKILHTAWH PSENIIAVAA421TNNLYIFQDK VNTRTRPLEQKLISEEDLAANDILDYKDDDDKV
432 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
