LSBio
Human PPP2R2B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human PPP2R2B Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all PPP2R2B antibodies →
$220.00each
Catalog Number: LS-G78365-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human PPP2R2B (NM_181676) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
B55BETA | PP2ABBETA | PP2APR55BETA | PP2A subunit B isoform beta | PR2AB55BETA | PR55-BETA | SCA12 | Spinocerebellar ataxia 12 | PP2A subunit B isoform R2-beta | PP2AB55BETA | PP2APR55B | PR55BETA | PPP2R2B | PR2ABBETA | PR2APR55BETA | PR52B
Origin Species
Human
NCBI GenBank
NM_004576
SwissProt
Q00005

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
51.8 kda
Protein Type
Lysate
Amino Acid Sequence
1MKCFSRYLPY IFRPPNTILS SSCHTEADII STVEFNHTGE LLATGDKGGR VVIFQREQES
61KNQVHRRGEY NVYSTFQSHE PEFDYLKSLE IEEKINKIRW LPQQNAAYFL LSTNDKTVKL
121WKVSERDKRP EGYNLKDEEG RLRDPATITT LRVPVLRPMD LMVEATPRRV FANAHTYHIN
181SISVNSDYET YMSADDLRIN LWNFEITNQS FNIVDIKPAN MEELTEVITA AEFHPHHCNT
241FVYSSSKGTI RLCDMRASAL CDRHTKFFEE PEDPSNRSFF SEIISSISDV KFSHSGRYIM
301TRDYLTVKVW DLNMENRPIE TYQVHDYLRS KLCSLYENDC IFDKFECVWN GSDSVIMTGS
361YNNFFRMFDR NTKRDVTLEA SRENSKPRAI LKPRKVCVGG KRRKDEISVD SLDFSKKILH
421TAWHPSENII AVAATNNLYI FQDKVN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

446 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.