PPARD / PPAR Delta Antibody
Catalog Number: LS-C334195
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Immunofluorescence, Western Blot
Reactivity: Human, Mouse
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human PPARD / PPAR Delta Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G77979-20
Catalog Number: LS-G77979
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human PPAR delta(PPARD) (NM_001171819) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- FAAR | NUCI | PPAR-beta | PPAR-delta | PPARD | NR1C2 | NUC1 | NUCII | Nuclear hormone receptor 1 | PPARB | PPAR Delta
- Origin Species
- Human
- NCBI GenBank
- NM_006238
- SwissProt
- Q03181
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 46.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MHQRDLSRSS SPPSLLDQLQ MGCDGASCGS LNMECRVCGD KASGFHYGVH ACEGCKGFFR61RTIRMKLEYE KCERSCKIQK KNRNKCQYCR FQKCLALGMS HNAIRFGRMP EAEKRKLVAG121LTANEGSQYN PQVADLKAFS KHIYNAYLKN FNMTKKKARS ILTGKASHTA PFVIHDIETL181WQAEKGLVWK QLVNGLPPYK EISVHVFYRC QCTTVETVRE LTEFAKSIPS FSSLFLNDQV241TLLKYGVHEA IFAMLASIVN KDGLLVANGS GFVTREFLRS LRKPFSDIIE PKFEFAVKFN301ALELDDSDLA LFIAAIILCG DRPGLMNVPR VEAIQDTILR ALEFHLQANH PDAQYLFPKL361LQKMADLRQL VTEHAQMMQR IKKTETETSL HPLLQEIYKD MYTRTRPLEQKLISEEDLAANDILDYKDDDDKV
402 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
