LSBio
IHC-plus™ PPARD / PPAR Delta Antibody

Anti-PPAR Delta antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

IHC-plus™ PPARD / PPAR Delta Antibody

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Mouse
Format:Liquid
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$526.00each
Catalog Number: LS-B45-50

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Overview

Description
PPAR Delta antibody LS-B45 is an unconjugated rabbit polyclonal antibody to PPAR Delta (PPARD) from mouse. It is reactive with mouse and rat. Validated for ELISA, IHC and WB. Cited in 1 publication.
Application
ELISA, IHC, IHC-P, WB
Reactivity
Ms
Predicted Reactivity
Rat
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
FAAR | NUCI | PPAR-beta | PPAR-delta | PPARD | NR1C2 | NUC1 | NUCII | Nuclear hormone receptor 1 | PPARB | PPAR Delta
Recommended Dilution: ELISA
1:30000 - 1:70000
Recommended Dilution: IHC-P
2.5 µg/ml
Recommended Dilution: WB
1:500 - 1:5000
UniProt Number
Q03181
SwissProt
Q03181

Target

Target
PPARD / PPAR Delta
Antigen Species
Mouse
Specificity
A BLAST analysis was used to suggest reactivity with this protein from mouse and rat sources based on 100% homology for the immunogen sequence. Cross-reactivity with PPAR delta protein from human, chimpanzee and rabbit may occur as this sequence shows 85% homology with the protein from these sources. Cross-reactivity with PPAR delta homologues from other sources has not been determined. No reactivity is expected against other subtypes of PPAR.
Epitope
A BLAST analysis was used to suggest reactivity with this protein from mouse and rat sources based on 100% homology for the immunogen sequence. Cross-reactivity with PPAR delta protein from human, chimpanzee and rabbit may occur as this sequence shows 85% homology with the protein from these sources. Cross-reactivity with PPAR delta homologues from other sources has not been determined. No reactivity is expected against other subtypes of PPAR.
Gene Name
PPARD

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
Anti-PPAR delta antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the amino terminus of mouse PPAR delta.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate, 0.15 M NaCl
Transcript profiling identifies dynamic gene expression patterns and an important role for Nrf2/Keap1 pathway in the developing mouse esophagus. Chen H, Li J, Li H, Hu Y, Tevebaugh W, Yamamoto M, Que J, Chen X. PloS one. 2012;7:e36504. PubMed: 22567161 PMC: PMC3342176

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