POLB / DNA Polymerase Beta Antibody
Catalog Number: LS-C22843-100
100 µg / $878.00
Antibody: Mouse Monoclonal
Applications: Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity: Amphibian (Other), Hamster, Human, Mouse, Rat
Format: Liquid
Human POLB / DNA Polymerase Beta Protein (Recombinant His)
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| Expression System | Escherichia coli (Prokaryotic) |
| Format: | Liquid |
$267.00each
Catalog Number: LS-G1408-20
Catalog Number: LS-G1408
For bulk orders or custom formulations:
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- Description
- DNA polymerase beta, also known as POLB, belongs to the DNA polymerase type-X family. In eukaryotic cells, DNA polymerase beta (POLB) performs base excision repair (BER) required for DNA maintenance, replication, recombination, and drug resistance. POLB has two distinct domains, the larger is the polymerase domain itself, whereas a small basic N-terminal domain contains an AP lyase activity that excises the abasic sugar-phosphate residue at the strand break. POLB fills single nucleotide gaps in DNA produced by the base excision repair pathway of mammalian cells. Overexpression of POLB, as found in some human tumors, could confer an increase in spontaneous mutagenesis. Recombinant human POLB protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Category
- Proteins & Peptides > Proteins > Recombinant Proteins
- Alternative Names
- DNA pol beta | Mutant DNA polymerase beta | POLB | DNA polymerase beta | DNA polymerase beta subunit
- Origin Species
- Human
- NCBI GenBank
- NM_002690|NP_002681.1
- SwissProt
- P06746
Protein
- Expression System
- Escherichia coli (Prokaryotic)
- Fusion Tag
- Hexahistidine Tag
- Molecular Weight
- 40.3 kDa
- Protein Type
- Recombinant
- Amino Acid Sequence
11-335aa|MG SSHHHHHHSS GLVPRGSHMS KRKAPQETLN GGITDMLTEL ANFEKNVSQA61IHKYNAYRKA ASVIAKYPHK IKSGAEAKKL PGVGTKIAEK IDEFLATGKL RKLEKIRQDD121TSSSINFLTR VSGIGPSAAR KFVDEGIKTL EDLRKNEDKL NHHQRIGLKY FGDFEKRIPR181EEMLQMQDIV LNEVKKVDSE YIATVCGSFR RGAESSGDMD VLLTHPSFTS ESTKQPKLLH241QVVEQLQKVH FITDTLSKGE TKFMGVCQLP SKNDEKEYPH RRIDIRLIPK DQYYCGVLYF301TGSDIFNKNM RAHALEKGFT INEYTIRPLG VTGVAGEPLP VDSEKDIFDY IQWKYREPKD361RSE
363 aa
Format
- Purity
- >90%
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 0.1M NaCl.
- Purity Measurement Method
- SDS-PAGE (Coomassie Stain)
Storage & Handling
- Storage Conditions
- Short term: store at 2°C to 8°C for up to 1 week. Long term: aliquot and store at -20°C to -80°C. Avoid freeze-thaw cycles.
