Polyclonal Rabbit anti-Human SLC5A11 / SMIT2 Antibody (WB)
Catalog Number: LS-C748223
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Immunohistochemistry (general), Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Western blot of Rabbit Anti-POL (DNA polymerase beta) Antibody (Anti-POLB (DNA polymerase beta) (RABBIT) Antibody - 600-401-C65). Lane 1: LN428 FLAG POL (control). Lane 2: A172. Load: 35 ug per lane. Primary antibody: POL antibody at 1:2000 for overnight at 4C. Secondary antibody: IRDye800 goat anti-rabbit at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~40 kDa, ~40 kDa. Other band(s): unknown band ~75 kDd.
Polyclonal Rabbit anti-Human POLB / DNA Polymerase Beta Antibody (C-Terminus, WB)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$513.00each
Catalog Number: LS-C153861-100
Catalog Number: LS-C153861
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- Description
- DNA Polymerase Beta antibody LS-C153861 is an unconjugated rabbit polyclonal antibody to human DNA Polymerase Beta (POLB) (C-Terminus). Validated for ELISA and WB. Cited in 2 publications.
- Application
- ELISA, IHC, WB
- Reactivity
- Hu
- Predicted Reactivity
- Bovine, Xenopus
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- DNA pol beta | Mutant DNA polymerase beta | POLB | DNA polymerase beta | DNA polymerase beta subunit
- Recommended Dilution: ELISA
- 1:50000
- UniProt Number
- P06746
- NCBI GenBank
- NM_002690 | NP_002681.1
- SwissProt
- P06746
Target
- Target
- Human POLB / DNA Polymerase Beta
- Antigen Species
- Human
- Epitope
- This antibody reacts with overexpressed and endogenous POLβ protein. A BLAST analysis was used to suggest reactivity with POLβ from human, bovine, and Xenopus laevis based on a 100% homology with the immunizing sequence. Cross-reactivity with POLβ from other sources has not been determined.
- Gene Name
- POLB
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminus of the POLβ protein.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
Regulated over-expression of DNA polymerase beta mediates early onset cataract in mice. Sobol RW, Foley JF, Nyska A, Davidson MG, Wilson SH. DNA repair. 2003;2:609-22.
PubMed: 12713817
DNA polymerase beta-dependent long patch base excision repair in living cells. Asagoshi K, Liu Y, Masaoka A, Lan L, Prasad R, Horton JK, Brown AR, Wang XH, Bdour HM, Sobol RW, Taylor JS, Yasui A, Wilson SH. DNA repair. 2010;9:109-19.
PubMed: 20006562 · PMC: PMC2819632
