LSBio
Human PIG1 / NUFIP2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human PIG1 / NUFIP2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all NUFIP2 antibodies →
$220.00each
Catalog Number: LS-G87515-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human NUFIP2 (NM_020772) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
82-kD FMRP Interacting Protein | 182-FIP | 82-FIP | FMRP-interacting protein 2 | FIP-82 | NUFIP2 | PIG1 | KIAA1321 | Proliferation-inducing gene 1
Origin Species
Human
NCBI GenBank
AB037742
SwissProt
Q7Z417

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
75.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEEKPGQPQP QHHHSHHHPH HHPQQQQQQP HHHHHYYFYN HSHNHHHHHH HQQPHQYLQH
61GAEGSPKAQP KPLKHEQKHT LQQHQETPKK KTGYGELNGN AGEREISLKN LSSDEATNPI
121SRVLNGNQQV VDTSLKQTVK ANTFGKAGIK TKNFIQKNSM DKKNGKSYEN KSGENQSVDK
181SDTIPIPNGV VTNNSGYITN GYMGKGADND GSGSESGYTT PKKRKARRNS AKGCENLNIV
241QDKIMQQETS VPTLKQGLET FKPDYSEQKG NRVDGSKPIW KYETGPGGTS RGKPAVGDML
301RKSSDSKPGV SSKKFDDRPK GKHASAVASK EDSWTLFKPP PVFPVDNSSA KIVPKISYAS
361KVKENLNKTI QNSSVSPTSS SSSSSSTGET QTQSSSRLSQ VPMSALKSVT SANFSNGPVL
421AGTDGNVYPP GGQPLLTTAA NTLTPISSGT DSVLQDMSLT SAAVEQIKTS LFIYPSNMQT
481MLLSTAQVDL PSQTDQQNLG DIFQNQWGLS FINEPSAGPE TVTGKSSEHK VMEVTFQGEY
541PATLVSQGAE IIPSGTEHPV FPKAYELEKR TSPQVLGSIL KSGTTSESGA LSLEPSHIGD
601LQKADTSSQG ALVFLSKDYE IESQNPLASP TNTLLGSAKE QRYQRGLERN DSWGSFDLRA
661AIVYHTKEME SIWNLQKQDP KRIITYNEAM DSPDQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

695 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.