Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PIG1 / NUFIP2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G111011-100
Catalog Number: LS-G111011
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NUFIP2 (NM_020772), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- 82-kD FMRP Interacting Protein | 182-FIP | 82-FIP | FMRP-interacting protein 2 | FIP-82 | NUFIP2 | PIG1 | KIAA1321 | Proliferation-inducing gene 1
- Origin Species
- Human
- NCBI GenBank
- AB037742
- SwissProt
- Q7Z417
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 75.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEEKPGQPQP QHHHSHHHPH HHPQQQQQQP HHHHHYYFYN HSHNHHHHHH HQQPHQYLQH61GAEGSPKAQP KPLKHEQKHT LQQHQETPKK KTGYGELNGN AGEREISLKN LSSDEATNPI121SRVLNGNQQV VDTSLKQTVK ANTFGKAGIK TKNFIQKNSM DKKNGKSYEN KSGENQSVDK181SDTIPIPNGV VTNNSGYITN GYMGKGADND GSGSESGYTT PKKRKARRNS AKGCENLNIV241QDKIMQQETS VPTLKQGLET FKPDYSEQKG NRVDGSKPIW KYETGPGGTS RGKPAVGDML301RKSSDSKPGV SSKKFDDRPK GKHASAVASK EDSWTLFKPP PVFPVDNSSA KIVPKISYAS361KVKENLNKTI QNSSVSPTSS SSSSSSTGET QTQSSSRLSQ VPMSALKSVT SANFSNGPVL421AGTDGNVYPP GGQPLLTTAA NTLTPISSGT DSVLQDMSLT SAAVEQIKTS LFIYPSNMQT481MLLSTAQVDL PSQTDQQNLG DIFQNQWGLS FINEPSAGPE TVTGKSSEHK VMEVTFQGEY541PATLVSQGAE IIPSGTEHPV FPKAYELEKR TSPQVLGSIL KSGTTSESGA LSLEPSHIGD601LQKADTSSQG ALVFLSKDYE IESQNPLASP TNTLLGSAKE QRYQRGLERN DSWGSFDLRA661AIVYHTKEME SIWNLQKQDP KRIITYNEAM DSPDQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
695 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.