PIAS3 Antibody
Catalog Number: LS-C346210
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Immunofluorescence, Immunohistochemistry (general), Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human PIAS3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G84006-20
Catalog Number: LS-G84006
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human PIAS3(PIAS3) (NM_006099) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- E3 SUMO-protein ligase PIAS3 | PIAS3 | ZMIZ5
- Origin Species
- Human
- NCBI GenBank
- NM_006099
- SwissProt
- Q9Y6X2
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 67.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MVMSFRVSEL QVLLGFAGRN KSGRKHELLA KALHLLKSSC APSVQMKIKE LYRRRFPRKT61LGPSDLSLLS LPPGTSPVGS PGPLAPIPPT LLAPGTLLGP KREVDMHPPL PQPVHPDVTM121KPLPFYEVYG ELIRPTTLAS TSSQRFEEAH FTFALTPQQV QQILTSREVL PGAKCDYTIQ181VQLRFCLCET SCPQEDYFPP NLFVKVNGKL CPLPGYLPPT KNGAEPKRPS RPINITPLAR241LSATVPNTIV VNWSSEFGRN YSLSVYLVRQ LTAGTLLQKL RAKGIRNPDH SRALIKEKLT301ADPDSEVATT SLRVSLMCPL GKMRLTVPCR ALTCAHLQSF DAALYLQMNE KKPTWTCPVC361DKKAPYESLI IDGLFMEILS SCSDCDEIQF MEDGSWCPMK PKKEASEVCP PPGYGLDGLQ421YSPVQGGDPS ENKKKVEVID LTIESSSDEE DLPPTKKHCS VTSAAIPALP GSKGVLTSGH481QPSSVLRSPA MGTLGGDFLS SLPLHEYPPA FPLGADIQGL DLFSFLQTES QHYGPSVITS541LDEQDALGHF FQYRGTPSHF LGPLAPTLGS SHCSATPAPP PGRVSSIVAP GGALREGHGG601PLPSGPSLTG CRSDIISLDTRTRPLEQKLISEEDLAANDILDYKDDDDKV
619 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
