LSBio
PIAS3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PIAS3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PIAS3 antibodies →
$504.00each
Catalog Number: LS-G100723-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PIAS3(PIAS3) (NM_006099), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
E3 SUMO-protein ligase PIAS3 | PIAS3 | ZMIZ5
Origin Species
Human
NCBI GenBank
NM_006099
SwissProt
Q9Y6X2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
67.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVMSFRVSEL QVLLGFAGRN KSGRKHELLA KALHLLKSSC APSVQMKIKE LYRRRFPRKT
61LGPSDLSLLS LPPGTSPVGS PGPLAPIPPT LLAPGTLLGP KREVDMHPPL PQPVHPDVTM
121KPLPFYEVYG ELIRPTTLAS TSSQRFEEAH FTFALTPQQV QQILTSREVL PGAKCDYTIQ
181VQLRFCLCET SCPQEDYFPP NLFVKVNGKL CPLPGYLPPT KNGAEPKRPS RPINITPLAR
241LSATVPNTIV VNWSSEFGRN YSLSVYLVRQ LTAGTLLQKL RAKGIRNPDH SRALIKEKLT
301ADPDSEVATT SLRVSLMCPL GKMRLTVPCR ALTCAHLQSF DAALYLQMNE KKPTWTCPVC
361DKKAPYESLI IDGLFMEILS SCSDCDEIQF MEDGSWCPMK PKKEASEVCP PPGYGLDGLQ
421YSPVQGGDPS ENKKKVEVID LTIESSSDEE DLPPTKKHCS VTSAAIPALP GSKGVLTSGH
481QPSSVLRSPA MGTLGGDFLS SLPLHEYPPA FPLGADIQGL DLFSFLQTES QHYGPSVITS
541LDEQDALGHF FQYRGTPSHF LGPLAPTLGS SHCSATPAPP PGRVSSIVAP GGALREGHGG
601PLPSGPSLTG CRSDIISLD
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

619 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.