PECAM-1 / CD31 Antibody (clone PECAM-1, FITC)
Catalog Number: LS-C783255-100
100 µg (0.2 mg/mL) / $322.00
Antibody: Mouse Monoclonal
Applications: Flow Cytometry
Reactivity: Human
Format: Liquid
Human PECAM-1 / CD31 Protein (Recombinant Fc, C-terminus)
ProteinLSBio Guarantee
| Expression System | Other |
| Format: | Lyophilized |
$283.00each
Catalog Number: LS-G137859-10
Catalog Number: LS-G137859
For bulk orders or custom formulations:
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- Description
- Platelet Endothelial Cell Adhesion Molecule (PECAM1, CD31), is a heavily glycosylated transmembrane protein belonging to the immunoglobulin (Ig) superfamily of cell adhesion molecules. CD31 is composed of an extracellular domain (ECD) of 574 amino acids (aa) containing six Ig-like domains, a transmembrane domain, and a 118 aa cytoplasmic domain. CD31 is highly expressed on endothelial cells and at a lower level on platelets, granulocytes, macrophages, dendritic cells, T and B cells, and natural killer (NK) cells. It is involved in cell adhesion and is required for transepithelial migration of leukocytes (TEM). CD31 acts as a homophilic receptor through its extracellular domain and is involved in downstream signaling via its cytoplasmic domain. This domain contains highly conserved ITIM motifs which, once tyrosine phosphorylated, recruit and activate the signaling molecules Src and SHP2. The resulting inhibition of TCR signaling increases the activation threshold of T cells, thus reinforcing peripheral tolerance and preventing development of autoimmunity. CD31 additionally regulates immune responses by acting as a key inhibitory receptor in dendritic cell development. CD31 is required for the transendothelial migration of leukocytes through intercellular junctions of vascular endothelial cells.
- Category
- Proteins & Peptides > Proteins > Recombinant Proteins
- Alternative Names
- CD31 | CD31 antigen | EndoCAM | PECA1 | PECAM1 | PECAM-1 | GPIIA
- Origin Species
- Hu
- NCBI GenBank
- NM_000442
- SwissProt
- P16284
Protein
- Expression System
- Other
- Fusion Tag
- hFc
- Protein Type
- Recombinant
- Amino Acid Sequence
1QENSFTINSV DMKSLPDWTV QNGKNLTLQC FADVSTTSHV KPQHQMLFYK DDVLFYNISS61MKSTESYFIP EVRIYDSGTY KCTVIVNNKE KTTAEYQVLV EGVPSPRVTL DKKEAIQGGI121VRVNCSVPEE KAPIHFTIEK LELNEKMVKL KREKNSRDQN FVILEFPVEE QDRVLSFRCQ181ARIISGIHMQ TSESTKSELV TVTESFSTPK FHISPTGMIM EGAQLHIKCT IQVTHLAQEF241PEIIIQKDKA IVAHNRHGNK AVYSVMAMVE HSGNYTCKVE SSRISKVSSI VVNITELFSK301PELESSFTHL DQGERLNLSC SIPGAPPANF TIQKEDTIVS QTQDFTKIAS KSDSGTYICT361AGIDKVVKKS NTVQIVVCEM LSQPRISYDA QFEVIKGQTI EVRCESISGT LPISYQLLKT421SKVLENSTKN SNDPAVFKDN PTEDVEYQCV ADNCHSHAKM LSEVLRVKVI APVDEVQISI481LSSKVVESGE DIVLQCAVNE GSGPITYKFY REKEGKPFYQ MTSNATQAFW TKQKANKEQE541GEYYCTAFNR ANHASSVPRS KILTVRVILA PWKKVDDIEG RMDEPKSCDK THTCPPCPAP601ELLGGPSVFL FPPKPKDTLM ISRTPEVTCV VVDVSHEDPE VKFNWYVDGV EVHNAKTKPR661EEQYNSTYRV VSVLTVLHQD WLNGKEYKCK VSNKALPAPI EKTISKAKGQ PREPQVYTLP721PSREEMTKNQ VSLTCLVKGF YPSDIAVEWE SNGQPENNYK TTPPVLDSDG SFFLYSKLTV781DKSRWQQGNV FSCSVMHEAL HNHYTQKSLS LSPGK
815 aa
Format
- Purity
- >95%
- Conjugate/Label
- Unconj
- Endotoxin Level
- < 1 EU/µg
- Format
- Lyophilized
- Formulation
- Lyophilized from a sterile solution in PBS, pH 7.4.
- Purity Measurement Method
- SDS-PAGE
Storage & Handling
- Storage Conditions
- Before reconstitution: short term; store at room temperature for up to 3 weeks, long term; store at -20°C. After Reconstitution: short term; store at 4°C to 7°C for up to 7 days, long term; Aliquot and store at -20°C for up to 3 months. Avoid freeze-thaw cycles.
