LSBio
Human NYAP1 / C7orf51 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human NYAP1 / C7orf51 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all NYAP1 antibodies →
$220.00each
Catalog Number: LS-G95934-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human C7orf51(NYAP1) (NM_173564) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_173564

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
87.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MNLLYRKTKL EWRQHKEEEA KRSSSKEVAP AGSAGPAAGQ GPGVRVRDIA SLRRSLRMGF
61MTMPASQEHT PHPCRSAMAP RSLSCHSVGS MDSVGGGPGG ASGGLTEDSS TRRPPAKPRR
121HPSTKLSMVG PGSGAETPPS KKAGSQKPTP EGRESSRKVP PQKPRRSPNT QLSVSFDESC
181PPGPSPRGGN LPLQRLTRGS RVAGDPDVGA QEEPVYIEMV GDVFRGGGRS GGGLAGPPLG
241GGGPTPPAGA DSDSEESEAI YEEMKYPLPE EAGEGRANGP PPLTATSPPQ QPHALPPHAH
301RRPASALPSR RDGTPTKTTP CEIPPPFPNL LQHRPPLLAF PQAKSASRTP GDGVSRLPVL
361CHSKEPAGST PAPQVPARER ETPPPPPPPP AANLLLLGPS GRARSHSTPL PPQGSGQPRG
421ERELPNSHSM ICPKAAGAPA APPAPAALLP GPPKDKAVSY TMVYSAVKVT THSVLPAGPP
481LGAGEPKTEK EISVLHGMLC TSSRPPVPGK TSPHGGAMGA AAGVLHHRGC LASPHSLPDP
541TVGPLTPLWT YPATAAGLKR PPAYESLKAG GVLNKGCGVG APSPMVKIQL QEQGTDGGAF
601ASISCAHVIA SAGTPEEEEE EVGAATFGAG WALQRKVLYG GRKAKELDKV EDGARAWNGS
661AEGPGKVERE DRGPGTSGIP VRSQGAEGLL ARIHHGDRGG SRTALPIPCQ TFPACHRNGD
721FTGGYRLGRS ASTSGVRQVV LHTPRPCSQP RDALSQPHPA LPLPLPLPPQ PARERDGKLL
781EVIERKRCVC KEIKARHRPD RGLCKQESMP ILPSWRRGPE PRKSGTPPCR RQHTVLWDTA
841I
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

841 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.