Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NYAP1 / C7orf51 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G105211-100
Catalog Number: LS-G105211
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C7orf51(NYAP1) (NM_173564), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Origin Species
- Human
- NCBI GenBank
- NM_173564
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 87.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MNLLYRKTKL EWRQHKEEEA KRSSSKEVAP AGSAGPAAGQ GPGVRVRDIA SLRRSLRMGF61MTMPASQEHT PHPCRSAMAP RSLSCHSVGS MDSVGGGPGG ASGGLTEDSS TRRPPAKPRR121HPSTKLSMVG PGSGAETPPS KKAGSQKPTP EGRESSRKVP PQKPRRSPNT QLSVSFDESC181PPGPSPRGGN LPLQRLTRGS RVAGDPDVGA QEEPVYIEMV GDVFRGGGRS GGGLAGPPLG241GGGPTPPAGA DSDSEESEAI YEEMKYPLPE EAGEGRANGP PPLTATSPPQ QPHALPPHAH301RRPASALPSR RDGTPTKTTP CEIPPPFPNL LQHRPPLLAF PQAKSASRTP GDGVSRLPVL361CHSKEPAGST PAPQVPARER ETPPPPPPPP AANLLLLGPS GRARSHSTPL PPQGSGQPRG421ERELPNSHSM ICPKAAGAPA APPAPAALLP GPPKDKAVSY TMVYSAVKVT THSVLPAGPP481LGAGEPKTEK EISVLHGMLC TSSRPPVPGK TSPHGGAMGA AAGVLHHRGC LASPHSLPDP541TVGPLTPLWT YPATAAGLKR PPAYESLKAG GVLNKGCGVG APSPMVKIQL QEQGTDGGAF601ASISCAHVIA SAGTPEEEEE EVGAATFGAG WALQRKVLYG GRKAKELDKV EDGARAWNGS661AEGPGKVERE DRGPGTSGIP VRSQGAEGLL ARIHHGDRGG SRTALPIPCQ TFPACHRNGD721FTGGYRLGRS ASTSGVRQVV LHTPRPCSQP RDALSQPHPA LPLPLPLPPQ PARERDGKLL781EVIERKRCVC KEIKARHRPD RGLCKQESMP ILPSWRRGPE PRKSGTPPCR RQHTVLWDTA841ITRTRPLEQKLISEEDLAANDILDYKDDDDKV
841 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.