LSBio
Human NR5A2 / LRH-1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human NR5A2 / LRH-1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all NR5A2 antibodies →
$220.00each
Catalog Number: LS-G80645-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human NR5A2(NR5A2) (NM_003822) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
B1F | B1F2 | B1-binding factor | CPF | CYP7A promoter-binding factor | FTZ-F1beta | FTF | Liver receptor homolog 1 | LRH1 | LRH-1 | HB1F | HB1F-2 | Nuclear receptor phr-1 | FTZ-F1 | Liver receptor homolog-1 | NR5A2 | Nuclear receptor NR5A2 | Receptor phr
Origin Species
Human
NCBI GenBank
NM_003822
SwissProt
O00482

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
56.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSSNSDTGDL QESLKHGLTP IVSQFKMVNY SYDEDLEELC PVCGDKVSGY HYGLLTCESC
61KGFFKRTVQN NKRYTCIENQ NCQIDKTQRK RCPYCRFQKC LSVGMKLEAV RADRMRGGRN
121KFGPMYKRDR ALKQQKKALI RANGLKLEAM SQVIQAMPSD LTISSAIQNI HSASKGLPLN
181HAALPPTDYD RSPFVTSPIS MTMPPHGSLQ GYQTHGHFPS RAIKSEYPDP YTSSPESIMG
241YSYMDSYQTS SPASIPHLIL ELLKCEPDEP QVQAKIMAYL QQEQANRSKH EKLSTFGLMC
301KMADQTLFSI VEWARSSIFF RELKVDDQMK LLQNCWSELL ILDHIYRQVV HGKEGSIFLV
361TGQQVDYSII ASQAGATLNN LMSHAQELVA KLRSLQFDQR EFVCLKFLVL FSLDVKNLEN
421FQLVEGVQEQ VNAALLDYTM CNYPQQTEKF GQLLLRLPEI RAISMQAEEY LYYKHLNGDV
481PYNNLLIEML HAKRA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

495 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.