Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NR5A2 / LRH-1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G100139-100
Catalog Number: LS-G100139
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NR5A2(NR5A2) (NM_003822), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- B1F | B1F2 | B1-binding factor | CPF | CYP7A promoter-binding factor | FTZ-F1beta | FTF | Liver receptor homolog 1 | LRH1 | LRH-1 | HB1F | HB1F-2 | Nuclear receptor phr-1 | FTZ-F1 | Liver receptor homolog-1 | NR5A2 | Nuclear receptor NR5A2 | Receptor phr
- Origin Species
- Hu
- NCBI GenBank
- NM_003822
- SwissProt
- O00482
Protein
- Expression System
- HEK293
- Fusion Tag
- FLAG, Myc
- Molecular Weight
- 56.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSSNSDTGDL QESLKHGLTP IVSQFKMVNY SYDEDLEELC PVCGDKVSGY HYGLLTCESC61KGFFKRTVQN NKRYTCIENQ NCQIDKTQRK RCPYCRFQKC LSVGMKLEAV RADRMRGGRN121KFGPMYKRDR ALKQQKKALI RANGLKLEAM SQVIQAMPSD LTISSAIQNI HSASKGLPLN181HAALPPTDYD RSPFVTSPIS MTMPPHGSLQ GYQTHGHFPS RAIKSEYPDP YTSSPESIMG241YSYMDSYQTS SPASIPHLIL ELLKCEPDEP QVQAKIMAYL QQEQANRSKH EKLSTFGLMC301KMADQTLFSI VEWARSSIFF RELKVDDQMK LLQNCWSELL ILDHIYRQVV HGKEGSIFLV361TGQQVDYSII ASQAGATLNN LMSHAQELVA KLRSLQFDQR EFVCLKFLVL FSLDVKNLEN421FQLVEGVQEQ VNAALLDYTM CNYPQQTEKF GQLLLRLPEI RAISMQAEEY LYYKHLNGDV481PYNNLLIEML HAKRATRTRPLEQKLISEEDLAANDILDYKDDDDKV
495 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconj
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.