LSBio
Human NPY4R / PPYR1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human NPY4R / PPYR1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all NPY4R antibodies →
$153.00each
Catalog Number: LS-G77677-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human NPY4R (NM_005972) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Neuropeptide Y4 receptor | Neuropeptide Y receptor type 4 | NPY4R | PP1 | PPYR1 | Y4 | NPY4-R
Origin Species
Human
NCBI GenBank
NM_005972
SwissProt
P50391

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
42 kDa
Protein Type
Lysate
Amino Acid Sequence
1MNTSHLLALL LPKSPQGENR SKPLGTPYNF SEHCQDSVDV MVFIVTSYSI ETVVGVLGNL
61CLMCVTVRQK EKANVTNLLI ANLAFSDFLM CLLCQPLTSV YTIMDYWIFG ETLCKMSAFI
121QCMSVTVSIL SLVLVALERH QLIINPTGWK PSISQAYLGI VLIWVIACVL SLPFLANSIL
181ENVFHKNHSK ALEFLADKVV CTESWPLAHH RTIYTTFLLL FQYCLPLGFI LVCYARIYRR
241LQRQGRVFHK GTYSLRAGHM KQVNVVLVVM VVAFAVLWLP LHVFNSLEDW HHEAIPICHG
301NLIFLVCHLL AMASTCVNPF IYGFLNTNFK KEIKALVLTC QQSAPLEESE HLPLSTVHTE
361VSKGSLRLSG RSNPI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

375 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.