NFIB Antibody
Catalog Number: LS-C488446-100
100 µL / $309.00
Antibody: Rabbit Polyclonal
Applications: Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human NFIB Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G80306-20
Catalog Number: LS-G80306
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human NFIB(NFIB) (NM_001190738) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CTF | HMGIC/NFIB | NF1-B | NFI-B | NFIB | NFIB3 | NFIB2 | Nuclear factor 1 B-type | Nuclear factor 1/B | Nuclear factor I-b2 | Nuclear factor I/B | TGGCA-binding protein | NF-I/B | NFI-RED
- Origin Species
- Human
- NCBI GenBank
- NM_005596
- SwissProt
- O00712
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 50.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MERIPVSVDF WVVCCAVLKC NPGIPKRMST LCFGFSDEFH PFIEALLPHV RAIAYTWFNL61QARKRKYFKK HEKRMSKDEE RAVKDELLSE KPEIKQKWAS RLLAKLRKDI RQEYREDFVL121TVTGKKHPCC VLSNPDQKGK IRRIDCLRQA DKVWRLDLVM VILFKGIPLE STDGERLMKS181PHCTNPALCV QPHHITVSVK ELDLFLAYYV QEQDSGQSGS PSHNDPAKNP PGYLEDSFVK241SGVFNVSELV RVSRTPITQG TGVNFPIGEI PSQPYYHDMN SGVNLQRSLS SPPSSKRPKT301ISIDENMEPS PTGDFYPSPS SPAAGSRTWH ERDQDMSSPT TMKKPEKPLF SSASPQDSSP361RLSTFPQHHH PGIPGVAHSV ISTRTPPPPS PLPFPTQAIL PPAPSSYFSH PTIRYPPHLN421PQDTLKNYVP SYDPSSPQTS QSWYLGTRTRPLEQKLISEEDLAANDILDYKDDDDKV
446 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
