LSBio
Human MYO19 / Myosin XIX Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human MYO19 / Myosin XIX Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all MYO19 antibodies →
$153.00each
Catalog Number: LS-G83789-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human MYO19 (NM_025109) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Myosin XIX | Myosin-XIX | MYO19 | MYOHD1 | Unconventional myosin-XIX
Origin Species
Human
NCBI GenBank
NM_025109
SwissProt
Q96H55

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
86.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLQQVNGHNP GSDGQAREYL REDLQEFLGG EVLLYKLDDL TRVNPVTLET VLRCLQARYM
61ADTFYTNAGC TLVALNPFKP VPQLYSPELM REYHAAPQPQ KLKPHVFTVG EQTYRNVKSL
121IEPVNQSIVV SGESGAGKTW TSRCLMKFYA VVATSPASWE SHKIAERIEQ RILNSNPVME
181AFGNACTLRN NNSSRFGKFI QLQLNRAQQM TGAAVQTYLL EKTRVACQAS SERNFHIFYQ
241ICKGASEDER LQWHLPEGAA FSWLPNPERS LEEDCFEVTR EAMLHLGIDT PTQNNIFKVL
301AGLLHLGNIQ FAASEDEAQP CQPMDDAKYS VRTAASLLGL PEDVLLEMVQ IRTIRAGRQQ
361QVFRKPCARA ECDTRRDCLA KLIYARLFDW LVSVINSSIC ADTDSWTTFI GLLDVYGFES
421FPDNSLEQLC INYANEKLQL EACGLVETIH ISAAGFPIRV SHRNFVERYK LLRRLHPCTS
481SGPDSPYPAK GLPEWCPHSE EATLEPLIQD ILHTLPVLTQ AAAITGDSAE AMPAPMHCGR
541TKVFMTDSML ELLECGRARV LEQCARCIQG GWRRHRHREQ ERQWRAVMLI QAAIRSWLTR
601KHIQRLHAAA TVIKRAWQKW RIRMACLAAK ELDGVEEKHF SQAPCSLSTS PLQTRLLEAI
661IRLWPLGLVL ANTAMGVGSF QRKLVVWACL QLPRGSPSSY TVQTAQDQAG VTSIRALPQG
721SIKFHCRKSP LRYADICPEP SPYSITGFNQ ILLERHRLIH VTSSAFTGLG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

770 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.