Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
MYO19 / Myosin XIX Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G109581-100
Catalog Number: LS-G109581
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MYO19 (NM_025109), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Myosin XIX | Myosin-XIX | MYO19 | MYOHD1 | Unconventional myosin-XIX
- Origin Species
- Human
- NCBI GenBank
- NM_025109
- SwissProt
- Q96H55
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 86.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MLQQVNGHNP GSDGQAREYL REDLQEFLGG EVLLYKLDDL TRVNPVTLET VLRCLQARYM61ADTFYTNAGC TLVALNPFKP VPQLYSPELM REYHAAPQPQ KLKPHVFTVG EQTYRNVKSL121IEPVNQSIVV SGESGAGKTW TSRCLMKFYA VVATSPASWE SHKIAERIEQ RILNSNPVME181AFGNACTLRN NNSSRFGKFI QLQLNRAQQM TGAAVQTYLL EKTRVACQAS SERNFHIFYQ241ICKGASEDER LQWHLPEGAA FSWLPNPERS LEEDCFEVTR EAMLHLGIDT PTQNNIFKVL301AGLLHLGNIQ FAASEDEAQP CQPMDDAKYS VRTAASLLGL PEDVLLEMVQ IRTIRAGRQQ361QVFRKPCARA ECDTRRDCLA KLIYARLFDW LVSVINSSIC ADTDSWTTFI GLLDVYGFES421FPDNSLEQLC INYANEKLQL EACGLVETIH ISAAGFPIRV SHRNFVERYK LLRRLHPCTS481SGPDSPYPAK GLPEWCPHSE EATLEPLIQD ILHTLPVLTQ AAAITGDSAE AMPAPMHCGR541TKVFMTDSML ELLECGRARV LEQCARCIQG GWRRHRHREQ ERQWRAVMLI QAAIRSWLTR601KHIQRLHAAA TVIKRAWQKW RIRMACLAAK ELDGVEEKHF SQAPCSLSTS PLQTRLLEAI661IRLWPLGLVL ANTAMGVGSF QRKLVVWACL QLPRGSPSSY TVQTAQDQAG VTSIRALPQG721SIKFHCRKSP LRYADICPEP SPYSITGFNQ ILLERHRLIH VTSSAFTGLGTRTRPLEQKLISEEDLAANDILDYKDDDDKV
770 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.