MKNK1 / MNK1 Antibody
Catalog Number: LS-C658755-100
100 µL / $394.00
Antibody: Rabbit Polyclonal
Applications: Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human MKNK1 / MNK1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G79692-20
Catalog Number: LS-G79692
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human MNK1(MKNK1) (NM_001135553) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- MKNK1 | MNK1 | MAPK-Interacting Kinase 1
- Origin Species
- Human
- NCBI GenBank
- NM_003684
- SwissProt
- Q9BUB5
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 47.2 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MVSSQKLEKP IEMGSSEPLP IADGDRRRKK KRRGRATDSL PGKFEDMYKL TSELLGEGAY61AKVQGAVSLQ NGKEYAVKII EKQAGHSRSR VFREVETLYQ CQGNKNILEL IEFFEDDTRF121YLVFEKLQGG SILAHIQKQK HFNEREASRV VRDVAAALDF LHTKGIAHRD LKPENILCES181PEKVSPVKIC DFDLGSGMKL NNSCTPITTP ELTTPCGSAE YMAPEVVEVF TDQATFYDKR241CDLWSLGVVL YIMLSGYPPF VGHCGADCGW DRGEVCRVCQ NKLFESIQEG KYEFPDKDWA301HISSEAKDLI SKLLVRDAKQ RLSAAQVLQH PWVQGQAPEK GLPTPQVLQR NSSTMDLTLF361AAEAIALNRQ LSQHEENELA EEPEALADGL CSMKLSPPCK SRLARRRALA QAGRGEDRSP421PTALTRTRPLEQKLISEEDLAANDILDYKDDDDKV
424 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
