Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
MKNK1 / MNK1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G117158-100
Catalog Number: LS-G117158
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MNK1(MKNK1) (NM_003684), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- MKNK1 | MNK1 | MAPK-Interacting Kinase 1
- Origin Species
- Human
- NCBI GenBank
- NM_003684
- SwissProt
- Q9BUB5
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 51.2 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MVSSQKLEKP IEMGSSEPLP IADGDRRRKK KRRGRATDSL PGKFEDMYKL TSELLGEGAY61AKVQGAVSLQ NGKEYAVKII EKQAGHSRSR VFREVETLYQ CQGNKNILEL IEFFEDDTRF121YLVFEKLQGG SILAHIQKQK HFNEREASRV VRDVAAALDF LHTKDKVSLC HLGWSAMAPS181GLTAAPTSLG SSDPPTSASQ VAGTTGIAHR DLKPENILCE SPEKVSPVKI CDFDLGSGMK241LNNSCTPITT PELTTPCGSA EYMAPEVVEV FTDQATFYDK RCDLWSLGVV LYIMLSGYPP301FVGHCGADCG WDRGEVCRVC QNKLFESIQE GKYEFPDKDW AHISSEAKDL ISKLLVRDAK361QRLSAAQVLQ HPWVQGQAPE KGLPTPQVLQ RNSSTMDLTL FAAEAIALNR QLSQHEENEL421AEEPEALADG LCSMKLSPPC KSRLARRRAL AQAGRGEDRS PPTALTRTRP LEQKLISEED481LAANDILDYK DDDDKV
496 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.