LSBio
Human KRT82 / Keratin 82 / KRTHB2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human KRT82 / Keratin 82 / KRTHB2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all KRT82 antibodies →
$220.00each
Catalog Number: LS-G92456-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human KRT82 (NM_033033) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Hard keratin | type II | 2 | HB2 | K82 | Keratin 82 | Keratin | hair | basic | KRTHB2 | type II cuticular Hb2 | Keratin-82 | KRT82 | Type II hair keratin Hb2 | Type-II keratin Kb22 | Hb-2
Origin Species
Human
SwissProt
Q9NSB4

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
56.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSYHSFQPGS RCGSQSFSSY SAVMPRMVTH YAVSKGPCRP GGGRGLRALG CLGSRSLCNV
61GFGRPRVASR CGGTLPGFGY RLGATCGPSA CITPVTINES LLVPLALEID PTVQRVKRDE
121KEQIKCLNNR FASFINKVRF LEQKNKLLET KWNFMQQQRC CQTNIEPIFE GYISALRRQL
181DCVSGDRVRL ESELCSLQAA LEGYKKKYEE ELSLRPCVEN EFVALKKDVD TAFLMKADLE
241TNAEALVQEI DFLKSLYEEE ICLLQSQISE TSVIVKMDNS RELDVDGIIA EIKAQYDDIA
301SRSKAEAEAW YQCRYEELRV TAGNHCDNLR NRKNEILEMN KLIQRLQQET ENVKAQRCKL
361EGAIAEAEQQ GEAALNDAKC KLAGLEEALQ KAKQDMACLL KEYQEVMNSK LGLDIEIATY
421RRLLEGEEHR LCEGIGPVNI SVSSSKGAFL YEPCGVSTPV LSTGVLRSNG GCSIVGTGEL
481YVPCEPQGLL SCGSGRKSSM TLGAGGSSPS HKH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

513 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.