Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KRT82 / Keratin 82 / KRTHB2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G108490-100
Catalog Number: LS-G108490
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KRT82 (NM_033033), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Hard keratin | type II | 2 | HB2 | K82 | Keratin 82 | Keratin | hair | basic | KRTHB2 | type II cuticular Hb2 | Keratin-82 | KRT82 | Type II hair keratin Hb2 | Type-II keratin Kb22 | Hb-2
- Origin Species
- Human
- SwissProt
- Q9NSB4
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 56.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSYHSFQPGS RCGSQSFSSY SAVMPRMVTH YAVSKGPCRP GGGRGLRALG CLGSRSLCNV61GFGRPRVASR CGGTLPGFGY RLGATCGPSA CITPVTINES LLVPLALEID PTVQRVKRDE121KEQIKCLNNR FASFINKVRF LEQKNKLLET KWNFMQQQRC CQTNIEPIFE GYISALRRQL181DCVSGDRVRL ESELCSLQAA LEGYKKKYEE ELSLRPCVEN EFVALKKDVD TAFLMKADLE241TNAEALVQEI DFLKSLYEEE ICLLQSQISE TSVIVKMDNS RELDVDGIIA EIKAQYDDIA301SRSKAEAEAW YQCRYEELRV TAGNHCDNLR NRKNEILEMN KLIQRLQQET ENVKAQRCKL361EGAIAEAEQQ GEAALNDAKC KLAGLEEALQ KAKQDMACLL KEYQEVMNSK LGLDIEIATY421RRLLEGEEHR LCEGIGPVNI SVSSSKGAFL YEPCGVSTPV LSTGVLRSNG GCSIVGTGEL481YVPCEPQGLL SCGSGRKSSM TLGAGGSSPS HKHTRTRPLEQKLISEEDLAANDILDYKDDDDKV
513 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.