LSBio
Human KRT78 / Keratin 78 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human KRT78 / Keratin 78 Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all KRT78 antibodies →
$220.00each
Catalog Number: LS-G95122-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human KRT78 (NM_173352) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CK-78 | Cytokeratin-78 | Kb40 | Keratin 5b | Keratin-78 | K5B | Keratin 78 | Keratin-5b | Type-II keratin Kb40 | K78 | KRT78
Origin Species
Human
NCBI GenBank
NM_173352
SwissProt
Q8N1N4

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
56.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSLSPCRAQR GFSARSACSA RSRGRSRGGF SSRGGFSSRS LNSFGGCLEG SRGSTWGSGG
61RLGVRFGEWS GGPGLSLCPP GGIQEVTINQ NLLTPLKIEI DPQFQVVRTQ ETQEIRTLNN
121QFASFIDKVR FLEQQNKVLE TKWHLLQQQG LSGSQQGLEP VFEACLDQLR KQLEQLQGER
181GALDAELKAC RDQEEEYKSK YEEEAHRRAT LENDFVVLKK DVDGVFLSKM ELEGKLEALR
241EYLYFLKHLN EEELGQLQTQ ASDTSVVLSM DNNRYLDFSS IITEVRARYE EIARSSKAEA
301EALYQTKYQE LQVSAQLHGD RMQETKVQIS QLHQEIQRLQ SQTENLKKQN ASLQAAITDA
361EQRGELALKD AQAKVDELEA ALRMAKQNLA RLLCEYQELT STKLSLDVEI ATYRRLLEGE
421ECRMSGECTS QVTISSVGGS AVMSGGVGGG LGSTCGLGSG KGSPGSCCTS IVTGGSNIIL
481GSGKDPVLDS CSVSGSSAGS SCHTILKKTV ESSLKTSITY
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

520 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.