Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KRT78 / Keratin 78 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G105356-100
Catalog Number: LS-G105356
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KRT78 (NM_173352), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CK-78 | Cytokeratin-78 | Kb40 | Keratin 5b | Keratin-78 | K5B | Keratin 78 | Keratin-5b | Type-II keratin Kb40 | K78 | KRT78
- Origin Species
- Human
- NCBI GenBank
- NM_173352
- SwissProt
- Q8N1N4
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 56.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSLSPCRAQR GFSARSACSA RSRGRSRGGF SSRGGFSSRS LNSFGGCLEG SRGSTWGSGG61RLGVRFGEWS GGPGLSLCPP GGIQEVTINQ NLLTPLKIEI DPQFQVVRTQ ETQEIRTLNN121QFASFIDKVR FLEQQNKVLE TKWHLLQQQG LSGSQQGLEP VFEACLDQLR KQLEQLQGER181GALDAELKAC RDQEEEYKSK YEEEAHRRAT LENDFVVLKK DVDGVFLSKM ELEGKLEALR241EYLYFLKHLN EEELGQLQTQ ASDTSVVLSM DNNRYLDFSS IITEVRARYE EIARSSKAEA301EALYQTKYQE LQVSAQLHGD RMQETKVQIS QLHQEIQRLQ SQTENLKKQN ASLQAAITDA361EQRGELALKD AQAKVDELEA ALRMAKQNLA RLLCEYQELT STKLSLDVEI ATYRRLLEGE421ECRMSGECTS QVTISSVGGS AVMSGGVGGG LGSTCGLGSG KGSPGSCCTS IVTGGSNIIL481GSGKDPVLDS CSVSGSSAGS SCHTILKKTV ESSLKTSITYTRTRPLEQKLISEEDLAANDILDYKDDDDKV
520 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.