KCNK10 / TREK Antibody
Catalog Number: LS-C747084
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human KCNK10 / TREK Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G91490-20
Catalog Number: LS-G91490
For bulk orders or custom formulations:
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human KCNK10 (NM_138317) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- K2p10.1 | KCNK10 | TREK-2 | TREK2 | TWIK-related K+ channel 2 | TREK-2 K(+) channel subunit | Potassium channel TREK-2
- Origin Species
- Human
- NCBI GenBank
- NM_021161
- SwissProt
- P57789
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 60.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKFPIETPRK QVNWDPKVAV PAAAPVCQPK SATNGQPPAP APTPTPRLSI SSRATVVARM61EGTSQGGLQT VMKWKTVVAI FVVVVVYLVT GGLVFRALEQ PFESSQKNTI ALEKAEFLRD121HVCVSPQELE TLIQHALDAD NAGVSPIGNS SNNSSHWDLG SAFFFAGTVI TTIGYGNIAP181STEGGKIFCI LYAIFGIPLF GFLLAGIGDQ LGTIFGKSIA RVEKVFRKKQ VSQTKIRVIS241TILFILAGCI VFVTIPAVIF KYIEGWTALE SIYFVVVTLT TVGFGDFVAG GNAGINYREW301YKPLVWFWIL VGLAYFAAVL SMIGDWLRVL SKKTKEEVGE IKAHAAEWKA NVTAEFRETR361RRLSVEIHDK LQRAATIRSM ERRRLGLDQR AHSLDMLSPE KRSVFAALDT GRFKASSQES421INNRPNNLRL KGPEQLNKHG QGASEDNIIN KFGSTSRLTK RKNKDLKKTL PEDVQKIYKT481FRNYSLDEEK KEEETEKMCN SDNSSTAMLT DCIQQHAELE NGMIPTDTKD REPENNSLLE541DRNTRTRPLEQKLISEEDLAANDILDYKDDDDKV
543 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
