Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KCNK10 / TREK Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G102232-100
Catalog Number: LS-G102232
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KCNK10 (NM_138317), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- K2p10.1 | KCNK10 | TREK-2 | TREK2 | TWIK-related K+ channel 2 | TREK-2 K(+) channel subunit | Potassium channel TREK-2
- Origin Species
- Human
- NCBI GenBank
- NM_021161
- SwissProt
- P57789
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 60.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKFPIETPRK QVNWDPKVAV PAAAPVCQPK SATNGQPPAP APTPTPRLSI SSRATVVARM61EGTSQGGLQT VMKWKTVVAI FVVVVVYLVT GGLVFRALEQ PFESSQKNTI ALEKAEFLRD121HVCVSPQELE TLIQHALDAD NAGVSPIGNS SNNSSHWDLG SAFFFAGTVI TTIGYGNIAP181STEGGKIFCI LYAIFGIPLF GFLLAGIGDQ LGTIFGKSIA RVEKVFRKKQ VSQTKIRVIS241TILFILAGCI VFVTIPAVIF KYIEGWTALE SIYFVVVTLT TVGFGDFVAG GNAGINYREW301YKPLVWFWIL VGLAYFAAVL SMIGDWLRVL SKKTKEEVGE IKAHAAEWKA NVTAEFRETR361RRLSVEIHDK LQRAATIRSM ERRRLGLDQR AHSLDMLSPE KRSVFAALDT GRFKASSQES421INNRPNNLRL KGPEQLNKHG QGASEDNIIN KFGSTSRLTK RKNKDLKKTL PEDVQKIYKT481FRNYSLDEEK KEEETEKMCN SDNSSTAMLT DCIQQHAELE NGMIPTDTKD REPENNSLLE541DRNTRTRPLEQKLISEEDLAANDILDYKDDDDKV
543 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.