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Human ITGAV/Integrin Alpha V/CD51 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human ITGAV/Integrin Alpha V/CD51 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
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$220.00each
Catalog Number: LS-G79563-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human CD51(ITGAV) (NM_001144999) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CD51 | CD51 antigen | Integrin | alpha V | MSK8 | Integrin Alpha V | Integrin alpha-V | ITGAV | VNRA | Vitronectin receptor | Integrin alphaVbeta3 | VTNR
Origin Species
Human
NCBI GenBank
NM_002210
SwissProt
P06756

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
111 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLLGTLLLIL YILMLCRMFL LVGAPKANTT QPGIVEGGQV LKCDWSSTRR CQPIEFDATG
61NRDYAKDDPL EFKSHQWFGA SVRSKQDKIL ACAPLYHWRT EMKQEREPVG TCFLQDGTKT
121VEYAPCRSQD IDADGQGFCQ GGFSIDFTKA DRVLLGGPGS FYWQGQLISD QVAEIVSKYD
181PNVYSIKYNN QLATRTAQAI FDDSYLGYSV AVGDFNGDGI DDFVSGVPRA ARTLGMVYIY
241DGKNMSSLYN FTGEQMAAYF GFSVAATDIN GDDYADVFIG APLFMDRGSD GKLQEVGQVS
301VSLQRASGDF QTTKLNGFEV FARFGSAIAP LGDLDQDGFN DIAIAAPYGG EDKKGIVYIF
361NGRSTGLNAV PSQILEGQWA ARSMPPSFGY SMKGATDIDK NGYPDLIVGA FGVDRAILYR
421ARPVITVNAG LEVYPSILNQ DNKTCSLPGT ALKVSCFNVR FCLKADGKGV LPRKLNFQVE
481LLLDKLKQKG AIRRALFLYS RSPSHSKNMT ISRGGLMQCE ELIAYLRDES EFRDKLTPIT
541IFMEYRLDYR TAADTTGLQP ILNQFTPANI SRQAHILLDC GEDNVCKPKL EVSVDSDQKK
601IYIGDDNPLT LIVKAQNQGE GAYEAELIVS IPLQADFIGV VRNNEALARL SCAFKTENQT
661RQVVCDLGNP MKAGTQLLAG LRFSVHQQSE MDTSVKFDLQ IQSSNLFDKV SPVVSHKVDL
721AVLAAVEIRG VSSPDHIFLP IPNWEHKENP ETEEDVGPVV QHIYELRNNG PSSFSKAMLH
781LQWPYKYNNN TLLYILHYDI DGPMNCTSDM EINPLRIKIS SLQTTEKNDT VAGQGERDHL
841ITKRDLALSE GDIHTLGCGV AQCLKIVCQV GRLDRGKSAI LYVKSLLWTE TFMNKENQNH
901SYSLKSSASF NVIEFPYKNL PIEDITNSTL VTTNVTWGIQ PAPMPVPVWV IILAVLAGLL
961LLAVLVFVMY RMGFFKRVRP PQEEQEREQL QPHENGEGNS ET
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1002 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.