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Human IRAK3 / IRAKM / IRAK-M Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human IRAK3 / IRAKM / IRAK-M Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all IRAK3 antibodies →
$153.00each
Catalog Number: LS-G89610-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human IRAKM(IRAK3) (NM_007199) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ASRT5 | IRAK M | IRAK3 | IRAK-3 | IL1RAK M | IRAK-M | IRAKM
Origin Species
Human
NCBI GenBank
NM_007199
SwissProt
Q9Y616

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
67.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAGNCGARGA LSAHTLLFDL PPALLGELCA VLDSCDGALG WRGLAERLSS SWLDVRHIEK
61YVDQGKSGTR ELLWSWAQKN KTIGDLLQVL QEMGHRRAIH LITNYGAVLS PSEKSYQEGG
121FPNILFKETA NVTVDNVLIP EHNEKGVLLK SSISFQNIIE GTRNFHKDFL IGEGEIFEVY
181RVEIQNLTYA VKLFKQEKKM QCKKHWKRFL SELEVLLLFH HPNILELAAY FTETEKFCLI
241YPYMRNGTLF DRLQCVGDTA PLPWHIRIGI LIGISKAIHY LHNVQPCSVI CGSISSANIL
301LDDQFQPKLT DFAMAHFRSH LEHQSCTINM TSSSSKHLWY MPEEYIRQGK LSIKTDVYSF
361GIVIMEVLTG CRVVLDDPKH IQLRDLLREL MEKRGLDSCL SFLDKKVPPC PRNFSAKLFC
421LAGRCAATRA KLRPSMDEVL NTLESTQASL YFAEDPPTSL KSFRCPSPLF LENVPSIPVE
481DDESQNNNLL PSDEGLRIDR MTQKTPFECS QSEVMFLSLD KKPESKRNEE ACNMPSSSCE
541ESWFPKYIVP SQDLRPYKVN IDPSSEAPGH SCRSRPVESS CSSKFSWDEY EQYKKE
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

596 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.