Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
IRAK3 / IRAKM / IRAK-M Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G125981-100
Catalog Number: LS-G125981
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human IRAKM(IRAK3) (NM_001142523), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ASRT5 | IRAK M | IRAK3 | IRAK-3 | IL1RAK M | IRAK-M | IRAKM
- Origin Species
- Human
- NCBI GenBank
- NM_007199
- SwissProt
- Q9Y616
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 60.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAGNCGARGA LSAHTLLFDL PPALLGELCA VLDSCDGALG WRGLGAVLSP SEKSYQEGGF61PNILFKETAN VTVDNVLIPE HNEKGILLKS SISFQNIIEG TRNFHKDFLI GEGEIFEVYR121VEIQNLTYAV KLFKQEKKMQ CKKHWKRFLS ELEVLLLFHH PNILELAAYF TETEKFCLIY181PYMRNGTLFD RLQCVGDTAP LPWHIRIGIL IGISKAIHYL HNVQPCSVIC GSISSANILL241DDQFQPKLTD FAMAHFRSHL EHQSCTINMT SSSSKHLWYM PEEYIRQGKL SIKTDVYSFG301IVIMEVLTGC RVVLDDPKHI QLRDLLRELM EKRGLDSCLS FLDKKVPPCP RNFSAKLFCL361AGRCAATRAK LRPSMDEVLN TLESTQASLY FAEDPPTSLK SFRCPSPLFL ENVPSIPVED421DESQNNNLLP SDEGLRIDRM TQKTPFECSQ SEVMFLSLDK KPESKRNEEA CNMPSSSCEE481SWFPKYIVPS QDLRPYKVNI DPSSEAPGHS CRSRPVESSC SSKFSWDEYE QYKKETRTRPLEQKLISEEDLAANDILDYKDDDDKV
535 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.