LSBio
Human IFFO / IFFO1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human IFFO / IFFO1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all IFFO1 antibodies →
$220.00each
Catalog Number: LS-G83532-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human IFFO(IFFO1) (NM_080730) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
IFFO | HOM-TES-103 | IFFO1 | Tumor antigen HOM-TES-103
Origin Species
Human
NCBI GenBank
BC001790

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
61.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MNPLFGPNLF LLQQEQQGLA GPLGDSLGGD HFAGGGDLPP APLSPAGPAA YSPPGPGPAP
61PAAMALRNDL GSNINVLKTL NLRFRCFLAK VHELERRNRL LEKQLQQALE EGKQGRRGLG
121RRDQAVQTGF VSPIRPLGLQ LGARPAAVCS PSARVLGSPA RSPAGPLAPS AASLSSSSTS
181TSTTYSSSAR FMPGTIWSFS HARRLGPGLE PTLVQGPGLS WVHPDGVGVQ IDTITPEIRA
241LYNVLAKVKR ERDEYKRRWE EEYTVRIQLQ DRVNELQEEA QEADACQEEL ALKVEQLKAE
301LVVFKGLMSN NLSELDTKIQ EKAMKVDMDI CRRIDITAKL CDVAQQRNCE DMIQMFQVPS
361MGGRKRERKA AVEEDTSLSE SEGPRQPDGD EEESTALSIN EEMQRMLNQL REYDFEDDCD
421SLTWEETEET LLLWEDFSGY AMAAAEAQGE QEDSLEKVIK DTESLFKTRE KEYQETIDQI
481ELELATAKND MNRHLHEYME MCSMKRGLDV QMETCRRLIT QSGDRKSPAF TAVPLSDPPP
541PPSEAEDSDR DVSSDSSMR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

559 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.