Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
IFFO / IFFO1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G107752-100
Catalog Number: LS-G107752
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human IFFO(IFFO1) (NM_080730), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- IFFO | HOM-TES-103 | IFFO1 | Tumor antigen HOM-TES-103
- Origin Species
- Human
- NCBI GenBank
- BC001790
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 61.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MNPLFGPNLF LLQQEQQGLA GPLGDSLGGD HFAGGGDLPP APLSPAGPAA YSPPGPGPAP61PAAMALRNDL GSNINVLKTL NLRFRCFLAK VHELERRNRL LEKQLQQALE EGKQGRRGLG121RRDQAVQTGF VSPIRPLGLQ LGARPAAVCS PSARVLGSPA RSPAGPLAPS AASLSSSSTS181TSTTYSSSAR FMPGTIWSFS HARRLGPGLE PTLVQGPGLS WVHPDGVGVQ IDTITPEIRA241LYNVLAKVKR ERDEYKRRWE EEYTVRIQLQ DRVNELQEEA QEADACQEEL ALKVEQLKAE301LVVFKGLMSN NLSELDTKIQ EKAMKVDMDI CRRIDITAKL CDVAQQRNCE DMIQMFQVPS361MGGRKRERKA AVEEDTSLSE SEGPRQPDGD EEESTALSIN EEMQRMLNQL REYDFEDDCD421SLTWEETEET LLLWEDFSGY AMAAAEAQGE QEDSLEKVIK DTESLFKTRE KEYQETIDQI481ELELATAKND MNRHLHEYME MCSMKRGLDV QMETCRRLIT QSGDRKSPAF TAVPLSDPPP541PPSEAEDSDR DVSSDSSMRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
559 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.