HTR3E / 5-HT3E Receptor Antibody
Catalog Number: LS-C810167
2 sizes / from $288.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human HTR3E / 5-HT3E Receptor Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G93687-20
Catalog Number: LS-G93687
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human 5HT3E(HTR3E) (NM_182589) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- 5-HT3c1 | 5-HT3E | 5-HT3-E | HTR3E | Serotonin receptor 3E
- Origin Species
- Human
- SwissProt
- A5X5Y0
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 52.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MLAFILSRAT PRPALGPLSY REHRVALLHL THSMSTTGRG VTFTINCSGF GQHGADPTTL61NSVFNRKPFR PVTNISVPTQ VNISFAMSAI LDVNEQLHLL SSFLWLEMVW DNPFISWNPE121ECEGITKMSM AAKNLWLPDI FIIELMDVDK TPKGLTAYVS NEGRIRYKKP MKVDSICNLD181IFYFPFDQQN CTLTFSSFLY TVDSMLLDME KEVWEITDAS RNILQTHGEW ELLGLSKATA241KLSRGGNLYD QIVFYVAIRR RPSLYVINLL VPSGFLVAID ALSFYLPVKS GNRVPFKITL301LLGYNVFLLM MSDLLPTSGT PLIGVYFALC LSLMVGSLLE TIFITHLLHV ATTQPPPLPR361WLHSLLLHCN SPGRCCPTAP QKENKGPGLT PTHLPGVKEP EVSAGQMPGP AEAELTGGSE421WTRAQREHEA QKQHSVELWL QFSHAMDAML FRLYLLFMAS SIITVICLWN TTRTRPLEQKLISEEDLAANDILDYKDDDDKV
471 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
