Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
HTR3E / 5-HT3E Receptor Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G104235-100
Catalog Number: LS-G104235
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human 5HT3E(HTR3E) (NM_182589), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- 5-HT3c1 | 5-HT3E | 5-HT3-E | HTR3E | Serotonin receptor 3E
- Origin Species
- Human
- SwissProt
- A5X5Y0
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 52.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MLAFILSRAT PRPALGPLSY REHRVALLHL THSMSTTGRG VTFTINCSGF GQHGADPTTL61NSVFNRKPFR PVTNISVPTQ VNISFAMSAI LDVNEQLHLL SSFLWLEMVW DNPFISWNPE121ECEGITKMSM AAKNLWLPDI FIIELMDVDK TPKGLTAYVS NEGRIRYKKP MKVDSICNLD181IFYFPFDQQN CTLTFSSFLY TVDSMLLDME KEVWEITDAS RNILQTHGEW ELLGLSKATA241KLSRGGNLYD QIVFYVAIRR RPSLYVINLL VPSGFLVAID ALSFYLPVKS GNRVPFKITL301LLGYNVFLLM MSDLLPTSGT PLIGVYFALC LSLMVGSLLE TIFITHLLHV ATTQPPPLPR361WLHSLLLHCN SPGRCCPTAP QKENKGPGLT PTHLPGVKEP EVSAGQMPGP AEAELTGGSE421WTRAQREHEA QKQHSVELWL QFSHAMDAML FRLYLLFMAS SIITVICLWN TTRTRPLEQKLISEEDLAANDILDYKDDDDKV
471 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.