HNF1B / HNF1 Beta Antibody
Catalog Number: LS-C402563
4 sizes / from $260.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general)
Reactivity: Human, Mouse, Rat
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human HNF1B / HNF1 Beta Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G76432-20
Catalog Number: LS-G76432
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human HNF1 beta(HNF1B) (NM_001165923) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- FJHN | HNF-1B | HNF1 homeobox B | HNF1B | HNF2 | HPC11 | LF-B3 | LFB3 | HNF1beta | TCF2 | MODY5 | VHNF1 | HNF-1-beta | HNF1 beta A | Homeoprotein LFB3 | TCF-2 | Transcription factor 2
- Origin Species
- Human
- NCBI GenBank
- NM_000458
- SwissProt
- P35680
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 58.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MVSKLTSLQQ ELLSALLSSG VTKEVLVQAL EELLPSPNFG VKLETLPLSP GSGAEPDTKP61VFHTLTNGHA KGRLSGDEGS EDGDDYDTPP ILKELQALNT EEAAEQRAEV DRMLSEDPWR121AAKMIKGYMQ QHNIPQREVV DVTGLNQSHL SQHLNKGTPM KTQKRAALYT WYVRKQREIL181RQFSQQSHGP GQSDDACSEP TNKKMRRNRF KWGPASQQIL YQAYDRQKNP SKEEREALVE241ECNRAECLQR GVSPSKAHGL GSNLVTEVRV YNWFANRRKE EAFRQKLAMD AYSSNQTHSL301NPLLSHGSPH HQPSSSPPNK LSGVRYSQQG NNEITSSSTI SHHGNSAMVT SQSVLQQVSP361ASLDPGHNLL SPDGKMISVS GGGLPPVSTL TNIHSLSHHN PQQSQNLIMT PLSGVMAIAQ421SLNTSQAQSV PVINSVAGSL AALQPVQFSQ QLHSPHQQPL MQQSPGSHMA QQPFMAAVTQ481LQNSHMYAHK QEPPQYSHTS RFPSAMVVTD TSSISTLTNM SSSKQCPLQA WTRTRPLEQKLISEEDLAANDILDYKDDDDKV
531 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
