HERC3 Antibody
Catalog Number: LS-C395183
2 sizes / from $300.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human HERC3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G76648-20
Catalog Number: LS-G76648
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human HERC3 (NM_014606) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HERC3 | Hect domain and RLD 3 | KIAA0032
- Origin Species
- Human
- NCBI GenBank
- D25215
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 117 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MLCWGYWSLG QPGISTNLQG IVAEPQVCGF ISDRSVKEVA CGGNHSVFLL EDGEVYTCGL61NTKGQLGHER EGNKPEQIGA LADQHIIHVA CGESHSLALS DRGQLFSWGA GSDGQLGLMT121TEDSVAVPRL IQKLNQQTIL QVSCGNWHCL ALAADGQFFT WGKNSHGQLG LGKEFPSQAS181PQRVRSLEGI PLAQVAAGGA HSFALSLSGA VFGWGMNNAG QLGLSDEKDR ESPCHVKLLR241TQKVVYISCG EEHTAVLTKS GGVFTFGAGS CGQLGHDSMN DEVNPRRVLE LMGSEVTQIA301CGRQHTLAFV PSSGLIYAFG CGARGQLGTG HTCNVKCPSP VKGYWAAHSG QLSARADRFK361YHIVKQIFSG GDQTFVLCSK YENYSPAVDF RTMNQAHYTS LINDETIAVW RQKLSEHNNA421NTINGVVQIL SSAACWNGSF LEKKIDEHFK TSPKIPGIDL NSTRVLFEKL MNSQHSMILE481QILNSFESCL IPQLSSSPPD VEAMRIYLIL PEFPLLQDSK YYITLTIPLA MAILRLDTNP541SKVLDNWWSQ VCPKYFMKLV NLYKGAVLYL LRGRKTFLIP VLFNNYITAA LKLLEKLYKV601NLKVKHVEYD TFYIPEISNL VDIQEDYLMW FLHQAGMKAR PSIIQDTVTL CSYPFIFDAQ661AKTKMLQTDA ELQMQVAVNG ANLQNVFMLL TLEPLLARSP FLVLHVRRNN LVGDALRELS721IHSDIDLKKP LKVIFDGEEA VDAGGVTKEF FLLLLKELLN PIYGMFTYYQ DSNLLWFSDT781CFVEHNWFHL IGITCGLAIY NSTVVDLHFP LALYKKLLNV KPGLEDLKEL SPTEGRSLQE841LLDYPGEDVE ETFCLNFTIC RESYGVIEQK KLIPGGDNVT VCKDNRQEFV DAYVNYVFQI901SVHEWYTAFS SGFLKVCGGK VLELFQPSEL RAMMVGNSNY NWEELQETAI YKGDYSATHP961TVKLFWETFH EFPLEKKKKF LLFLTGSDRI PIYGMASLQI VIQSTASGEE YLPVAHTCYN1021LLDLPKYSSK EILSARLTQA LDNYEGFSLATRTRPLEQKLISEEDLAANDILDYKDDDDKV
1050 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
