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HERC3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

HERC3 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G113826-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HERC3 (NM_014606), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HERC3 | Hect domain and RLD 3 | KIAA0032
Origin Species
Human
NCBI GenBank
D25215

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
117 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLCWGYWSLG QPGISTNLQG IVAEPQVCGF ISDRSVKEVA CGGNHSVFLL EDGEVYTCGL
61NTKGQLGHER EGNKPEQIGA LADQHIIHVA CGESHSLALS DRGQLFSWGA GSDGQLGLMT
121TEDSVAVPRL IQKLNQQTIL QVSCGNWHCL ALAADGQFFT WGKNSHGQLG LGKEFPSQAS
181PQRVRSLEGI PLAQVAAGGA HSFALSLSGA VFGWGMNNAG QLGLSDEKDR ESPCHVKLLR
241TQKVVYISCG EEHTAVLTKS GGVFTFGAGS CGQLGHDSMN DEVNPRRVLE LMGSEVTQIA
301CGRQHTLAFV PSSGLIYAFG CGARGQLGTG HTCNVKCPSP VKGYWAAHSG QLSARADRFK
361YHIVKQIFSG GDQTFVLCSK YENYSPAVDF RTMNQAHYTS LINDETIAVW RQKLSEHNNA
421NTINGVVQIL SSAACWNGSF LEKKIDEHFK TSPKIPGIDL NSTRVLFEKL MNSQHSMILE
481QILNSFESCL IPQLSSSPPD VEAMRIYLIL PEFPLLQDSK YYITLTIPLA MAILRLDTNP
541SKVLDNWWSQ VCPKYFMKLV NLYKGAVLYL LRGRKTFLIP VLFNNYITAA LKLLEKLYKV
601NLKVKHVEYD TFYIPEISNL VDIQEDYLMW FLHQAGMKAR PSIIQDTVTL CSYPFIFDAQ
661AKTKMLQTDA ELQMQVAVNG ANLQNVFMLL TLEPLLARSP FLVLHVRRNN LVGDALRELS
721IHSDIDLKKP LKVIFDGEEA VDAGGVTKEF FLLLLKELLN PIYGMFTYYQ DSNLLWFSDT
781CFVEHNWFHL IGITCGLAIY NSTVVDLHFP LALYKKLLNV KPGLEDLKEL SPTEGRSLQE
841LLDYPGEDVE ETFCLNFTIC RESYGVIEQK KLIPGGDNVT VCKDNRQEFV DAYVNYVFQI
901SVHEWYTAFS SGFLKVCGGK VLELFQPSEL RAMMVGNSNY NWEELQETAI YKGDYSATHP
961TVKLFWETFH EFPLEKKKKF LLFLTGSDRI PIYGMASLQI VIQSTASGEE YLPVAHTCYN
1021LLDLPKYSSK EILSARLTQA LDNYEGFSLA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1050 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.