HEG1 Protein (Over-Expression Lysate Myc-DDK (Flag))
Catalog Number: LS-G111046-100
100 µg (1 mg/mL) / $725.00
Expression System Human Embryonic Kidney 293
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human HEG1 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G95678-20
Catalog Number: LS-G95678
For bulk orders or custom formulations:
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human HEG1 (NM_020733) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Origin Species
- Human
- NCBI GenBank
- NM_020733
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 147.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MASPRASRWP PPLLLLLLPL LLLPPAAPGT RDPPPSPARR ALSLAPLAGA GLELQLERRP61EREPPPTPPR ERRGPATPGP SYRAPEPGAA TQRGPSGRAP RGGSADAAWK HWPESNTEAH121VENITFYQNQ EDFSTVSSKE GVMVQTSGKS HAASDAPENL TLLAETADAR GRSGSSSRTN181FTILPVGYSL EIATALTSQS GNLASESLHL PSSSSEFDER IAAFQTKSGT ASEMGTERAM241GLSEEWTVHS QEATTSAWSP SFLPALEMGE LTTPSRKRNS SGPDLSWLHF YRTAASSPLL301DLSSSSESTE KLNNSTGLQS SSVSQTKTMH VATVFTDGGP RTLRSLTVSL GPVSKTEGFP361KDSRIATTSS SVLLSPSAVE SRRNSRVTGN PGDEEFIEPS TENEFGLTSL RWQNDSPTFG421EHQLASSSEV QNGSPMSQTE TVSRSVAPMR GGEITAHWLL TNSTTSADVT GSSASYPEGV481NASVLTQFSD STVQSGGSHT ALGDRSYSES SSTSSSESLN SSAPRGERSI AGISYGQVRG541TAIEQRTSSD HTDHTYLSST FTKGERALLS ITDNSSSSDI VESSTSYIKI SNSSHSEYSS601FFHAQTERSN ISSYDGEYAQ PSTESPVLHT SNLPSYTPTI NMPNTSVVLD TDAEFVSDSS661SSSSSSSSSS SSGPPLPLPS VSQSHHLFSS ILPSTRASVH LLKSTSDAST PWSSSPSPLP721VSLTTSTSAP LSVSQTTLPQ SSSTPVLPRA RETPVTSFQT STMTSFMTML HSSQTADLKS781QSTPHQEKVI TESKSPSLVS LPTESTKAVT TNSPLPPSLT ESSTEQTLPA TSTNLAQMSP841TFTTTILKTS QPLMTTPGTL SSTASLVTGP IAVQTTAGKQ LSLTHPEILV PQISTEGGIS901TERNRVIVDA TTGLIPLTSV PTSAKEMTTK LGVTAEYSPA SRSLGTSPSP QTTVVSTAED961LAPKSATFAV QSSTQSPTTV SSSASVNSCA VNPCLHNGEC VADNTSRGYH CRCPPSWQGD1021DCSVDVNECL SNPCPSTAMC NNTQGSFICK CPVGYQLEKG ICNLVRTFVT EFKLKRTFLN1081TTVEKHSDLQ EVENEITKTL NMCFSALPSY IRSTVHASRE SNAVVISLQT TFSLASNVTL1141FDLADRMQKC VNSCKSSAEV CQLLGSQRRI FRAGSLCKRK SPECDKDTSI CTDLDGVALC1201QCKSGYFQFN KMDHSCRACE DGYRLENETC MSCPFGLGGL NCGNPYQLIT VVIAAAGGGL1261LLILGIALIV TCCRKNKNDI SKLIFKSGDF QMSPYAEYPK NPRSQEWGRE AIEMHENGST1321KNLLQMTDVY YSPTSVRNPE LERNGLYPAY TGLPGSRHSC IFPGQYNPSF ISDESRRRDY1381FTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1381 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
