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HEG1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

HEG1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all HEG1 antibodies →
$725.00each
Catalog Number: LS-G111046-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HEG1 (NM_020733), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_020733

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
147.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MASPRASRWP PPLLLLLLPL LLLPPAAPGT RDPPPSPARR ALSLAPLAGA GLELQLERRP
61EREPPPTPPR ERRGPATPGP SYRAPEPGAA TQRGPSGRAP RGGSADAAWK HWPESNTEAH
121VENITFYQNQ EDFSTVSSKE GVMVQTSGKS HAASDAPENL TLLAETADAR GRSGSSSRTN
181FTILPVGYSL EIATALTSQS GNLASESLHL PSSSSEFDER IAAFQTKSGT ASEMGTERAM
241GLSEEWTVHS QEATTSAWSP SFLPALEMGE LTTPSRKRNS SGPDLSWLHF YRTAASSPLL
301DLSSSSESTE KLNNSTGLQS SSVSQTKTMH VATVFTDGGP RTLRSLTVSL GPVSKTEGFP
361KDSRIATTSS SVLLSPSAVE SRRNSRVTGN PGDEEFIEPS TENEFGLTSL RWQNDSPTFG
421EHQLASSSEV QNGSPMSQTE TVSRSVAPMR GGEITAHWLL TNSTTSADVT GSSASYPEGV
481NASVLTQFSD STVQSGGSHT ALGDRSYSES SSTSSSESLN SSAPRGERSI AGISYGQVRG
541TAIEQRTSSD HTDHTYLSST FTKGERALLS ITDNSSSSDI VESSTSYIKI SNSSHSEYSS
601FFHAQTERSN ISSYDGEYAQ PSTESPVLHT SNLPSYTPTI NMPNTSVVLD TDAEFVSDSS
661SSSSSSSSSS SSGPPLPLPS VSQSHHLFSS ILPSTRASVH LLKSTSDAST PWSSSPSPLP
721VSLTTSTSAP LSVSQTTLPQ SSSTPVLPRA RETPVTSFQT STMTSFMTML HSSQTADLKS
781QSTPHQEKVI TESKSPSLVS LPTESTKAVT TNSPLPPSLT ESSTEQTLPA TSTNLAQMSP
841TFTTTILKTS QPLMTTPGTL SSTASLVTGP IAVQTTAGKQ LSLTHPEILV PQISTEGGIS
901TERNRVIVDA TTGLIPLTSV PTSAKEMTTK LGVTAEYSPA SRSLGTSPSP QTTVVSTAED
961LAPKSATFAV QSSTQSPTTV SSSASVNSCA VNPCLHNGEC VADNTSRGYH CRCPPSWQGD
1021DCSVDVNECL SNPCPSTAMC NNTQGSFICK CPVGYQLEKG ICNLVRTFVT EFKLKRTFLN
1081TTVEKHSDLQ EVENEITKTL NMCFSALPSY IRSTVHASRE SNAVVISLQT TFSLASNVTL
1141FDLADRMQKC VNSCKSSAEV CQLLGSQRRI FRAGSLCKRK SPECDKDTSI CTDLDGVALC
1201QCKSGYFQFN KMDHSCRACE DGYRLENETC MSCPFGLGGL NCGNPYQLIT VVIAAAGGGL
1261LLILGIALIV TCCRKNKNDI SKLIFKSGDF QMSPYAEYPK NPRSQEWGRE AIEMHENGST
1321KNLLQMTDVY YSPTSVRNPE LERNGLYPAY TGLPGSRHSC IFPGQYNPSF ISDESRRRDY
1381F
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1381 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.